Bontempi E, Cazzulo J J
Instituto Nacional de Diagnóstico e Investigación de la Enfermedad de Chagas Dr. Mario Fatala Chaben, Misteria de Salud y Acción Social, Buenos Aires, Argentina.
FEMS Microbiol Lett. 1990 Aug;58(3):337-41. doi: 10.1111/j.1574-6968.1990.tb14000.x.
The major cysteine proteinase (cruzipain) from Trypanosoma cruzi was able to digest human IgG, as shown by polyacrylamide gel electrophoresis in the presence of SDS, and by gel filtration on a Superose 12 column, in a FPLC system. The Fab fragment of IgG was only slightly degraded, but Fc was extensively hydrolyzed to small peptides. The results suggest that cruzipain might be involved in the defense mechanisms of the parasite against the immune response of the host.
克氏锥虫的主要半胱氨酸蛋白酶（克氏锥虫蛋白酶）能够消化人免疫球蛋白G，这在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳以及在快速蛋白质液相色谱系统中使用Superose 12柱进行的凝胶过滤实验中得到了证实。免疫球蛋白G的Fab片段仅轻微降解，但Fc被广泛水解为小肽。结果表明，克氏锥虫蛋白酶可能参与了寄生虫抵御宿主免疫反应的防御机制。
