Dipartimento di Chimica, Sapienza Università di Roma, Roma, Italia.
Anal Bioanal Chem. 2013 Jan;405(2-3):635-45. doi: 10.1007/s00216-011-5691-y. Epub 2012 Jan 25.
A shotgun proteomics approach was used to compare human plasma protein binding capability with cationic liposomes, DNA-cationic lipid complexes (lipoplexes), and lipid-polycation-DNA (LPD) complexes. Nano-high-performance liquid chromatography coupled with a high-resolution LTQ Orbitrap XL mass spectrometer was used to characterize and compare their protein corona. Spectral counting and area under curve methods were used to perform label-free quantification. Substantial qualitative and quantitative differences were found among proteins bound to the three different systems investigated. Protein variety found on lipoplexes and LPD complexes was richer than that found on cationic liposomes. There were also significant differences between the amounts of protein. Such results could help in the design of gene-delivery systems, because some proteins could be more selectively bound rather than others, and their bio-distribution could be driven in vivo for more efficient and effective gene therapy.
采用 shotgun 蛋白质组学方法比较了人血浆蛋白与阳离子脂质体、DNA-阳离子脂质复合物（脂质体）和脂质-聚阳离子-DNA（LPD）复合物的结合能力。使用纳米高效液相色谱与高分辨率 LTQ Orbitrap XL 质谱仪对其蛋白质冠进行了表征和比较。采用谱计数和曲线下面积方法进行无标记定量。在与三种不同系统结合的蛋白质之间发现了大量的定性和定量差异。脂质体和 LPD 复合物上发现的蛋白质种类比阳离子脂质体上的更丰富。蛋白的量也有显著差异。这些结果可能有助于基因传递系统的设计，因为一些蛋白质可能比其他蛋白质更具选择性地结合，并且它们的生物分布可以在体内进行驱动，以实现更高效和有效的基因治疗。
