Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
Bioresour Technol. 2012 Mar;108:149-54. doi: 10.1016/j.biortech.2011.12.143. Epub 2012 Jan 8.
This study investigated the use of acid hydrolyzed algae to enhance the enzymatic hydrolysis of lignocellulosic biomass. The farm-waste grown algal samples were first characterized, and the optimal conditions for algal hydrolysis using dilute sulfuric acid were determined. Neutralized algal hydrolysate was then tested as a reaction medium (replacing the pH buffer solution) for the enzymatic hydrolysis of a lignocellulose, alkali treated anaerobically-digested fiber. Our results showed that net glucose yields from enzymatic hydrolyses containing undiluted algal hydrolysate were at least 65% higher than net glucose yields from control media (bovine serum albumin solution, citrate buffer or distilled water). It is likely that the increase in net glucose yield is due, in part, to the binding of hydrolyzed algal proteins to lignin, which protects cellulase from binding to lignin. This study demonstrates a potential approach of using wastewater-grown algae as a co-substrate to significantly enhance the enzymatic hydrolysis of lignocellulosic materials.
本研究探讨了酸解藻类用于增强木质纤维素生物质的酶水解作用。首先对农场废物生长的藻类样本进行了表征，并确定了使用稀硫酸进行藻类水解的最佳条件。然后将中和的藻类水解物用作酶解的反应介质（替代 pH 缓冲溶液），用于酶解经碱处理的厌氧消化纤维。我们的结果表明，含有未稀释藻类水解物的酶解物的净葡萄糖产率至少比对照介质（牛血清白蛋白溶液、柠檬酸盐缓冲液或蒸馏水）的净葡萄糖产率高 65%。净葡萄糖产率的增加可能部分归因于水解藻类蛋白与木质素的结合，这保护了纤维素酶不与木质素结合。本研究证明了一种利用废水藻类作为共底物的潜在方法，可显著提高木质纤维素材料的酶水解作用。
