School of Geography and Environment, Jiangxi Normal University, Nanchang, 330022, China.
Water Sci Technol. 2012;65(4):703-12. doi: 10.2166/wst.2012.886.
Microbial communities in a coastal aquifer in the Zhuhai region of southern China were investigated by culture-independent molecular approaches. Four 16S rRNA gene libraries of three groundwater samples from varied depths and one seawater sample were constructed and analysed by the amplified ribosomal DNA restriction analysis technique (ARDRA). The phylogenetic analysis indicated that the 16S rDNA of clones presenting dominant ARDRA patterns were most similar to Proteobacteria, Bacteroidetes, Actinobacteria, Planctomycetes, and candidate divisions OPx (such as OP3, OP8, and OP11). In samples extracted from wells of 5-, 20-, and 35-m depth (i.e., D1, D5, and D6) Proteobacteria made up 32.3, 34.3, and 46.7% of the microbial communities, respectively. The same samples from D1, D5, and D6 also consisted of 5.0, 11.2, and 6.5% Bacteroidetes and 5.4, 6.6, and 7.8% Actinobacteria, respectively. In contrast, the seawater clone library had a predominant number of Proteobacteria (32.8%), while Bacteroidetes and Planctomycetes both accounted for 9.3%. Total microbial diversity remained relatively constant over the top layer to a depth of approximately 35 m, significant community vertical and horizontal (seawater-groundwater) shifts were observed for certain bacterial populations.
采用非培养的分子生物学方法研究了中国南部珠海市沿海含水层中的微生物群落。从不同深度的三个地下水样本和一个海水样本中构建了四个 16S rRNA 基因文库，并通过扩增核糖体 DNA 限制分析技术（ARDRA）进行了分析。系统发育分析表明，具有优势 ARDRA 模式的克隆 16S rDNA 与变形菌门、拟杆菌门、放线菌门、浮霉菌门和候选门 OPx（如 OP3、OP8 和 OP11）最为相似。在取自 5m、20m 和 35m 深度井（即 D1、D5 和 D6）的样本中，变形菌分别占微生物群落的 32.3%、34.3%和 46.7%。D1、D5 和 D6 中的相同样本还分别包含 5.0%、11.2%和 6.5%的拟杆菌门和 5.4%、6.6%和 7.8%的放线菌门。相比之下，海水克隆文库中变形菌的数量占优势（32.8%），而拟杆菌门和浮霉菌门均占 9.3%。从表层到约 35m 深处，微生物多样性基本保持稳定，某些细菌种群在垂直和水平方向（海水-地下水）发生了显著的群落变化。
