Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden/Wuhan Institute of Botany, Chinese Academy of Sciences, Lumo street, Wuhan 430074, Hubei, PR China.
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden/Wuhan Institute of Botany, Chinese Academy of Sciences, Lumo street, Wuhan 430074, Hubei, PR China.
Ecotoxicol Environ Saf. 2012 May;79:153-162. doi: 10.1016/j.ecoenv.2011.12.011. Epub 2012 Jan 25.
Perennial ryegrass (Lolium perenne L.) is a popular turfgrass species. To understand the molecular mechanisms of salinity tolerance, a suppression subtractive cDNA library was constructed for a salinity-tolerant ryegrass accession, with NaCl-treated (255 mM) plants as the tester. Differentially expressed cDNA fragments were cloned and screened. BLAST search revealed that 268 clones exhibited significant homologies to known genes. These genes could be categorized into 11 different functional groups, including metabolism, energy transfer, detoxification, compatible solute, cellular transport, transcription, signal transduction, etc. The salinity-regulated expression of selected genes was confirmed by RT-PCR analysis. The results suggested that these putatively salinity up-regulated genes may play a vital role in the salinity tolerance of perennial ryegrass. They can be used as candidate genes for creating stress-tolerant grasses and for understanding molecular mechanisms of plant adaptation to salinity stress.
多年生黑麦草（Lolium perenne L.）是一种受欢迎的草坪草种。为了了解耐盐性的分子机制，构建了一个耐盐性黑麦草品系的抑制性消减 cDNA 文库，以盐处理（255mM）的植物作为测试者。克隆和筛选差异表达的 cDNA 片段。BLAST 搜索表明，268 个克隆与已知基因具有显著的同源性。这些基因可以分为 11 个不同的功能组，包括代谢、能量转移、解毒、相容性溶质、细胞运输、转录、信号转导等。通过 RT-PCR 分析证实了所选基因的盐调控表达。结果表明，这些推测的盐上调基因可能在多年生黑麦草的耐盐性中发挥重要作用。它们可以作为创造抗逆性草的候选基因，并用于理解植物适应盐胁迫的分子机制。
