Kojima M, Yoshida A, Ishiko S, Tanaka K
Central Laboratory for Research and Education, Asahikawa Medical College, Japan.
Hokkaido Igaku Zasshi. 1990 Jul;65(4):419-27.
Permeability of the blood-retinal barrier (BRB) can be determined by vitreous fluorophotometry and computer analysis. In this study, we characterize our eye model and apply it to normal human subjects. In this investigation of BRB permeability, we employed four parameters for the eye model: the inward permeability (Pin) and outward permeability (Pout) of the BRB; the diffusion coefficient in the posterior vitreous gel (D-p); and the plasma fluorescein concentration. The values of Pin, D-p, and plasma fluorescein concentration significantly affected the fluorescein concentration in the posterior vitreous 60 minutes after intravenous fluorescein injection. The value of Pout had little influence on the fluorescein kinetics in the posterior vitreous 60 minutes after injection. The results of experiments on humans indicate that the estimated values obtained by simulation fit satisfactorily the measured values. It is suggested that our computer simulation method in conjunction with vitreous fluorophotometry can effectively estimate permeability of BRB in normal and pathological eyes.
血视网膜屏障（BRB）的通透性可通过玻璃体荧光光度测定法和计算机分析来确定。在本研究中，我们对我们的眼部模型进行了表征并将其应用于正常人类受试者。在这项关于BRB通透性的研究中，我们为眼部模型采用了四个参数：BRB的内向通透性（Pin）和外向通透性（Pout）；玻璃体后凝胶中的扩散系数（D-p）；以及血浆荧光素浓度。静脉注射荧光素60分钟后，Pin、D-p和血浆荧光素浓度的值对玻璃体后部的荧光素浓度有显著影响。注射后60分钟，Pout的值对玻璃体后部的荧光素动力学影响很小。人体实验结果表明，通过模拟获得的估计值与测量值拟合良好。建议我们的计算机模拟方法结合玻璃体荧光光度测定法可有效估计正常和病理眼中BRB的通透性。
