Chahal P S, Chowienczyk P J, Kohner E M
Invest Ophthalmol Vis Sci. 1985 Jul;26(7):977-82.
Fluorescein penetration into the posterior vitreous depends on plasma-free fluorescein concentration and blood-retinal barrier (BRB) permeability. The reproducibility of two methods of deriving BRB permeability was studied in 19 normal eyes of 14 subjects using vitreous fluorophotometry on two separate occasions. Plasma-free fluorescence was measured at intervals over 1 hr and posterior vitreous fluorescence was measured before (background scan), within 6 min (bolus) and at 60 min (measurement) after intravenous fluorescein (14 mg X kg-1). A computer algorithm subtracted background fluorescence from the measurement scan which was then corrected for signal spread by using a "spread" function derived from the bolus scan. BRB permeability coefficient and vitreous diffusion coefficients were derived by fitting a mathematical model to the plasma and corrected vitreous fluorescence data. A permeability index was also calculated by dividing the area under the vitreous fluorescence by the area under the plasma fluorescence curve. There were no significant differences in the results between right and left eyes. Mean +/- SD values on first and second occasions for all eyes were permeability coefficient: (1.91 +/- 0.94) and (2.08 +/- 0.95) X 10(-7) cm X s-1; diffusion coefficient: (1.33 +/- 0.68) and (1.19 +/- 0.54) X 10(-5) cm2 X s-1; and permeability index: (2.05 +/- 1.03) and (2.11 +/- 1.02) X 10(-7) cm X s-1.(ABSTRACT TRUNCATED AT 250 WORDS)
荧光素渗入玻璃体后段取决于无血浆荧光素浓度和血视网膜屏障（BRB）的通透性。在14名受试者的19只正常眼中，分两次使用玻璃体荧光光度法研究了两种推导BRB通透性方法的可重复性。在1小时内间隔测量无血浆荧光，在静脉注射荧光素（14mg·kg-1）前（背景扫描）、6分钟内（团注）和60分钟时（测量）测量玻璃体后段荧光。一种计算机算法从测量扫描中减去背景荧光，然后使用从团注扫描得出的“扩散”函数对信号扩散进行校正。通过将数学模型拟合到血浆和校正后的玻璃体荧光数据来推导BRB通透性系数和玻璃体扩散系数。还通过将玻璃体荧光曲线下的面积除以血浆荧光曲线下的面积来计算通透性指数。左右眼结果无显著差异。所有眼睛第一次和第二次测量的平均±标准差数值分别为：通透性系数：（1.91±0.94）和（2.08±0.95）×10-7cm·s-1；扩散系数：（1.33±0.68）和（1.19±0.54）×10-5cm2·s-1；通透性指数：（2.05±1.03）和（2.11±1.02）×10-7cm·s-1。（摘要截短于250字）
