McClane B A, McDonel J L
J Cell Physiol. 1979 May;99(2):191-200. doi: 10.1002/jcp.1040990205.
Vero (African green monkey kidney) cells grown in tissue culture monolayer were sensitive to Clostridium perfringens enterotoxin. Within 30 minutes of exposure to the enterotoxin gross morphological damage was observed and within 40 minutes approximately 75% of the cells had detached. Nearly half of the cells were nonviable following 35 to 40 minutes incubation with the enterotoxin. Doses as low as 0.1 ng caused small but detectable inhibition of plating efficiency of the cells while more than 100 ng caused the inhibition to approach 100%. Total inhibition of DNA, RNA, and protein synthesis occurred within 30 minutes exposure to enterotoxin. Heat inactivated enterotoxin had no apparent effects upon cellular morphology, detachment, viability, plating efficiency, or incorporation. We propose that the enterotoxin induces structural damage to the cytoplasmic membrane which results in loss of electrolytes and other essential substances from the cells. The outcome of this process is shut down of macromolecular synthesis, gross morphological damage, and eventual death of the cell.
在组织培养单层中生长的Vero（非洲绿猴肾）细胞对产气荚膜梭菌肠毒素敏感。暴露于肠毒素30分钟内可观察到明显的形态学损伤，40分钟内约75%的细胞已脱落。与肠毒素孵育35至40分钟后，近一半的细胞无法存活。低至0.1纳克的剂量会对细胞的平板接种效率产生微小但可检测到的抑制作用，而超过100纳克的剂量会使抑制率接近100%。暴露于肠毒素30分钟内，DNA、RNA和蛋白质合成完全受到抑制。热灭活的肠毒素对细胞形态、脱落、活力、平板接种效率或掺入没有明显影响。我们认为肠毒素会诱导细胞质膜的结构损伤，导致细胞内电解质和其他必需物质流失。这个过程的结果是大分子合成停止、明显的形态学损伤以及细胞最终死亡。
