Vladimirov Iu A, Proskurina E V, Izmaĭlov D Iu
Biofizika. 2011 Nov-Dec;56(6):1081-90.
The review gives concepts of analysis of kinetics of complex reactions with the participation of free-radicals. The concepts are based on the comparison of the kinetic curves of the chemiluminescent reactions in the presence of a physical enhancer coumarin C-525 with the simulated kinetic functions. This method was applied to the investigation of the mechanism of a branched-chain reaction of lipid peroxidation in biological and phospholipid membranes as well as to the effect of antioxidants and determination of their activities. It was also used for studying the reaction of the formation of free radicals in the complex of cytochrome c with cardiolipin. This reaction plays a key role in the initiation of apoptosis.
该综述给出了涉及自由基的复杂反应动力学分析的概念。这些概念基于在物理增强剂香豆素C - 525存在下化学发光反应的动力学曲线与模拟动力学函数的比较。该方法被应用于研究生物膜和磷脂膜中脂质过氧化支链反应的机制，以及抗氧化剂的作用及其活性的测定。它还被用于研究细胞色素c与心磷脂复合物中自由基形成的反应。该反应在细胞凋亡的启动中起关键作用。
