Coyle P, Rofe A M, Bourgeois C S, Conyers R A
Division of Clinical Chemistry, Institute of Medical and Veterinary Science, Adelaide, Australia.
Immunol Cell Biol. 1990 Jun;68 ( Pt 3):147-53. doi: 10.1038/icb.1990.21.
The physical and metabolic characteristics of a Dark Agouti rat mammary adenocarcinoma and its effects on host metabolism are described. The tumour was characterized by a lack of glandular differentiation, tetraploidy, a rapid mitotic index and a high rate of glycolysis. The adenocarcinoma was readily maintained in tissue culture and could be passaged through the host by inoculating either cell suspensions or tissue explants. In the rat, tumour growth resulted in a loss of adipose tissue at a tumour mass of less than 5% body weight indicating that increased energy expenditure was already present at that stage. In addition the tumour caused anaemia, hypercalcaemia and hypoglycaemia. Hyperketonaemia was also observed in fasted tumour-bearing rats. Methotrexate arrested tumour growth in vivo. These aspects of the tumour model make it useful for investigations into host-tumour competition and mechanisms of cachexia.
描述了一只深色刺豚鼠乳腺腺癌的物理和代谢特征及其对宿主代谢的影响。该肿瘤的特征是缺乏腺分化、四倍体、有丝分裂指数高和糖酵解速率高。这种腺癌很容易在组织培养中维持，并且可以通过接种细胞悬液或组织外植体在宿主体内传代。在大鼠中，肿瘤生长导致肿瘤质量小于体重的5%时脂肪组织减少，这表明在那个阶段能量消耗已经增加。此外，肿瘤还导致贫血、高钙血症和低血糖。在禁食的荷瘤大鼠中也观察到高酮血症。甲氨蝶呤在体内可阻止肿瘤生长。该肿瘤模型的这些方面使其对于研究宿主与肿瘤的竞争以及恶病质机制很有用。
