Department of Pharmacology, Sree Siddaganga College of Pharmacy, Tumkur 572 102, Karnataka, India.
J Ethnopharmacol. 2012 Mar 27;140(2):247-54. doi: 10.1016/j.jep.2012.01.012. Epub 2012 Jan 18.
In the traditional Indian and Thai system of medicine, Mimusops elengi Linn., flower is used as brain tonic and to calm anxiety and panic attacks.
The present study was designed to investigate the neuroprotective effect of hydroalcoholic extract of Mimusops elengi (ME) against cerebral ischemic reperfusion injury in rats.
Male rats were pretreated with ME (100 and 200mg/kg) for seven days and focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) method. After 60min of MCAO and 24h of reperfusion, a battery of behavioral tests assessed the extent of neurological deficits. Infarct volume and brain edema were measured in TTC stained brain sections and the extent of blood brain barrier (BBB) disruption was observed by Evan's blue extravasation. Oxidative and nitrative stress parameters were estimated in the brain homogenates. Further, simultaneous quantification of five polyphenolic biomarkers were done using HPLC.
Pretreatment with ME at doses of 100 and 200mg/kg significantly improved the neurobehavioral alterations and reduced the infarct volume, edema and extent of BBB disruption induced by ischemia reperfusion injury. It also prevented the alteration in the antioxidant status and reduced the nitrite levels when compared to ischemic animals. Further, HPLC studies revealed that ME contains five bioactive polyphenolic compounds.
These results clearly indicate the neuroprotective effect of ME against stroke like injury. The observed protective effect might be attributed to the polyphenolic compounds and their antioxidant and anti-inflammatory property.
在传统的印度和泰国医学体系中，Mimusops elengi Linn. 的花被用作补脑剂，用于镇静焦虑和恐慌发作。
本研究旨在研究 Mimusops elengi（ME）水醇提取物对大鼠脑缺血再灌注损伤的神经保护作用。
雄性大鼠用 ME（100 和 200mg/kg）预处理七天，通过大脑中动脉闭塞（MCAO）方法诱导局灶性脑缺血。MCAO 60min 后和再灌注 24h 后，一系列行为测试评估神经功能缺损程度。在 TTC 染色的脑切片中测量梗死体积和脑水肿，并通过 Evan's 蓝渗出观察血脑屏障（BBB）破坏的程度。在脑匀浆中评估氧化和硝化应激参数。进一步使用 HPLC 同时定量五种多酚生物标志物。
100 和 200mg/kg 的 ME 预处理可显著改善缺血再灌注损伤引起的神经行为改变，减少梗死体积、水肿和 BBB 破坏程度。与缺血动物相比，它还防止了抗氧化状态的改变和亚硝酸盐水平的降低。此外，HPLC 研究表明 ME 含有五种生物活性多酚化合物。
这些结果清楚地表明 ME 对中风样损伤具有神经保护作用。观察到的保护作用可能归因于多酚化合物及其抗氧化和抗炎特性。
