Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy and Gas Hydrate, Chinese Academy of Sciences, Guangzhou 510640, China.
Bioresour Technol. 2012 Mar;108:252-7. doi: 10.1016/j.biortech.2011.12.092. Epub 2011 Dec 24.
A laboratory set-up was designed to carry out high consistency enzymatic saccharification of sweet sorghum bagasse (SSB) which was pretreated by liquid hot water (LHW). The effects of two impellers on enzymatic hydrolysis of SSB were investigated. Compared with the double-curved-blade impeller (DCBI), the plate-and-frame impeller (PFI) could improve glucose production by 10%. Tween80 and fed-batch hydrolysis method adopted in this study produced total sugar of 17.06 g/L more than batch hydrolysis and raised the substrate consistency to 30%. At the final substrate loading of 30%, the concentrations of cellobiose, glucose and xylose reached to 15.01 g/L, 88.95 g/L and 9.80 g/L, respectively, and the ethanol concentration reached to 43.36 g/L in the case of cellobiose and xylose were not fermented by Saccharomyces cerevisiae Y2034. This study is an attempt at improvement of enzyme hydrolyzing LHW-pretreated material at high consistency.
设计了一个实验室装置，以对经液体热预处理的甜高粱渣进行高固酶解。考察了两种搅拌器对 SSB 酶解的影响。与双曲叶片搅拌器（DCBI）相比，板式框搅拌器（PFI）可将葡萄糖产量提高 10%。本文采用吐温 80 和分批补料水解法，比分批水解多生产 17.06 g/L 的总糖，将底物浓度提高到 30%。在最终底物负荷为 30%的情况下，如果酿酒酵母 Y2034 不发酵纤维二糖和木糖，纤维二糖、葡萄糖和木糖的浓度分别达到 15.01 g/L、88.95 g/L 和 9.80 g/L，乙醇浓度达到 43.36 g/L。本研究旨在提高酶解高浓度液体热预处理物料的效果。
