Provincial Key Laboratory of Agricultural Environmental Engineering, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan, PR China.
Bioresour Technol. 2011 Oct;102(19):8945-51. doi: 10.1016/j.biortech.2011.07.028. Epub 2011 Jul 21.
Steam-pretreated sweet sorghum bagasse (SSB) and Douglas-fir (DF) were employed for SHF and SSF to evaluate the effects of xylanase supplementation and delignification on ethanol production. Results indicated final ethanol concentration in SHF could reach 28.4 g/L (SSB) and 20.4 g/L (DF) by xylanase supplementation with the increase of 46% and 61% in comparison with controls. The delignification could significantly enhance final ethanol concentration to 31.2g/L (SSB) and 30.2 g/L (DF) with the increase of 61% and 138%. In SSF, final ethanol concentration in the delignified SSB and DF arrived at 27.6 g/L and 34.3 g/L with the increase of 26% and 157% compared with controls. However, only 2.2 g/L (SSB) and 6.9 g/L (DF) ethanol were obtained with xylanase supplementation. According to these results, it could be concluded that delignification was beneficial to improve ethanol production of SHF and SSF. The xylanase supplementation (0.12 g protein/g glucan) was only positive to SHF while retarded SSF seriously.
蒸汽预处理甜高粱渣（SSB）和花旗松被用于同步糖化发酵（SSF）和固态发酵（SHF），以评估木聚糖酶添加和脱木质素对乙醇生产的影响。结果表明，通过添加木聚糖酶，SSF 中 SSB 和 DF 的最终乙醇浓度可分别达到 28.4g/L 和 20.4g/L，与对照相比分别提高了 46%和 61%。脱木质素可显著提高最终乙醇浓度，SSB 和 DF 的最终乙醇浓度分别达到 31.2g/L 和 30.2g/L，分别提高了 61%和 138%。在 SSF 中，脱木质素 SSB 和 DF 的最终乙醇浓度分别达到 27.6g/L 和 34.3g/L，与对照相比分别提高了 26%和 157%。然而，添加木聚糖酶仅获得了 2.2g/L（SSB）和 6.9g/L（DF）乙醇。根据这些结果，可以得出结论，脱木质素有利于提高 SHF 和 SSF 的乙醇产量。木聚糖酶添加（0.12g 蛋白质/g 葡聚糖）对 SSF 仅呈正作用，而对 SSF 严重抑制。
