School of Chemical Engineering, Wuhan Textile University, Wuhan 430073, China.
J Chromatogr A. 2012 Mar 2;1227:253-8. doi: 10.1016/j.chroma.2011.12.105. Epub 2012 Jan 9.
Numerous micropumps have been developed, but few of them can produce adequate flow rate and pressure for high-performance liquid chromatography (HPLC) applications. We have recently developed an innovative hybrid electroosmotic pump (EOP) to solve this problem. The basic unit of a hybrid pump consists of a +EOP (the pumping element is positively charged) and a -EOP (the pumping element is negatively charged). The outlet of the +EOP is then joined with the inlet of the -EOP, forming a basic pump unit, while the anode of a positive high voltage (HV) power supply is placed at the joint. The inlet and outlet of this pump unit are electrically grounded. With this configuration, we can stack many of such pump units in series to boost the pumping power. In this work, we describe in details how an open-capillary hybrid EOP is constructed and characterize this pump systematically. We also show that a hybrid EOP with ten serially stacked pump units can deliver a maximum pressure of 21.5 MPa (∼3100 psi). We further demonstrate the feasibility of using this hybrid EOP to drive eluents for HPLC separations of proteins and peptides.
已经开发出了许多微泵，但它们很少能够为高效液相色谱 (HPLC) 应用提供足够的流速和压力。我们最近开发了一种创新的混合电渗流泵 (EOP) 来解决这个问题。混合泵的基本单元由一个 +EOP（泵送元件带正电荷）和一个 -EOP（泵送元件带负电荷）组成。然后，+EOP 的出口与 -EOP 的入口相连，形成一个基本的泵单元，而正高压 (HV) 电源的阳极则放置在连接处。该泵单元的入口和出口都接地。通过这种配置，我们可以将许多这样的泵单元串联起来以提高泵送功率。在这项工作中，我们详细描述了如何构建开放式毛细管混合 EOP，并对该泵进行了系统的特性描述。我们还表明，具有十个串联堆叠泵单元的混合 EOP 可以提供 21.5 MPa（约 3100 psi）的最大压力。我们进一步证明了使用这种混合 EOP 驱动蛋白质和肽的 HPLC 分离的洗脱液的可行性。
