Genetics Department, University College of Swansea, Singleton Park, Swansea SA2 8PP, UK.
Carcinogenesis. 1980 Jan;1(1):9-13. doi: 10.1093/carcin/1.1.9.
DNA replication and repair has been studied in human cells irradiated with single or split doses of UV at 254nm. A dose of 2 J.m(-2) followed by incubation in medium for periods of 12 to 48 h before a second irradiation with 5 or 10 J.m(-2) actually reduces the amount of DNA replication seen after the latter dose alone. Such a preirradiation did not perceptibly alter the rate of excision repair seen after the latter dose when measured by the incidence of UV endonuclease sensitive sites, 3H-bromodeoxyuridine incorporation, or the incidence of cytosine arabinoside induced breaks. Furthermore, the first two parameters were measured in molecules replicating or remaining unreplicated after the latter irradiation. No difference in repair rates was seen between these two classes of molecules.
已经研究了在 254nm 的紫外线单次或分割剂量照射下的人类细胞中的 DNA 复制和修复。在第二次用 5 或 10 J.m(-2) 照射之前，用 2 J.m(-2) 的剂量照射，然后在培养基中孵育 12 至 48 小时，实际上会减少在后者单独剂量后观察到的 DNA 复制量。这种预照射不会在后者剂量后通过紫外线内切酶敏感位点、3H-溴脱氧尿苷掺入或胞嘧啶阿拉伯糖苷诱导的断裂的发生率测量时，明显改变切除修复的速率。此外，后两个参数是在后者照射后复制或未复制的分子中测量的。在这两类分子之间没有观察到修复速率的差异。
