Institut Curie, Laboratoire Curie, 11, Rue Pierre et Marie Curie, 75231 Paris, Cedex 05, France.
Carcinogenesis. 1980 Jan;1(1):33-6. doi: 10.1093/carcin/1.1.33.
The fluorescence spectral properties of the principal hydrocarbon-nucleoside adducts present in hydrolysates of DNA that had been isolated from hamster embryo cells or from mouse skin treated with benz(a)anthracene have been examined using photon-counting spectrophotofluorimetry and compared with the spectral properties of the products formed when different diol-epoxides derived from benz(a)anthracene react with nucleic acid in solution. The adducts were separated by chromatography on LH20 Sephadex columns and purified by high pressure liquid chromatography. The spectra obtained are phenanthrene-like and not anthracene-like, a result that is consistent with metabolic activation occurring, through the formation of a vicinal diol-epoxide, in the 8,9,10,11-ring, rather than in the 1,2,3,4-ring of this hydrocarbon.
已使用光子计数分光荧光光度法检查了从用苯并(a)蒽处理的仓鼠胚胎细胞或从鼠皮中分离出的 DNA 水解物中存在的主要烃-核苷加合物的荧光光谱特性，并将其与不同二羟-环氧化物衍生自苯并(a)蒽与溶液中核酸反应时形成的产物的光谱特性进行了比较。加合物通过 LH20 葡聚糖柱色谱分离，并通过高压液相色谱进行纯化。所得光谱类似于菲而不是蒽，这一结果与代谢活化一致，代谢活化通过形成顺式二羟-环氧化物发生在该烃的 8、9、10、11 环中，而不是在 1、2、3、4 环中。
