Bártek J, Bártková J, Vojtĕsek B, Stasková Z, Rejthar A, Kovarík J, Lane D P
Research Institute of Clinical and Experimental Oncology, Brno, Czechoslovakia.
Int J Cancer. 1990 Nov 15;46(5):839-44. doi: 10.1002/ijc.2910460515.
An extensive series of histological sections reflecting the various states of normal breast tissue, and a range of benign and malignant lesions, were examined for the expression of the p53 protein using a panel of anti-p53 antibodies. In 2 separate series the results of using frozen or methacarn-fixed, paraffin-embedded sections were compared. Strong positive staining for p53 was detected in over 50% of the malignant lesions when frozen sections were used. This number fell to just over 20% when methacarn-fixed sections were examined. In neither series was any p53 staining seen in normal breast or in the benign lesions. Studies by Western blotting on breast cell lines confirmed that this histological signal is due to a pronounced over-expression of the p53 protein. Earlier studies show that this over-expression is associated with mutation of the p53 gene. Mutation of the p53 gene with over-expression of the mutant protein is therefore one of the most frequent specific genetic changes in malignant breast cancer.
使用一组抗p53抗体，对一系列反映正常乳腺组织不同状态以及一系列良性和恶性病变的组织学切片进行p53蛋白表达检测。在两个独立的系列中，对使用冷冻切片或甲醇 Carnoy 固定、石蜡包埋切片的结果进行了比较。当使用冷冻切片时，超过50%的恶性病变中检测到p53强阳性染色。当检查甲醇 Carnoy 固定切片时，这一数字降至略高于20%。在这两个系列中，正常乳腺或良性病变中均未见到任何p53染色。对乳腺细胞系进行的蛋白质印迹研究证实，这种组织学信号是由于p53蛋白明显过度表达所致。早期研究表明，这种过度表达与p53基因突变有关。因此，p53基因突变伴突变蛋白过度表达是恶性乳腺癌中最常见的特定基因变化之一。
