Pischel K D, Little J R
J Immunol. 1979 Aug;123(2):551-6.
A simple technique is presented for the identification of particular cell membrane antigens. The method employs labeled membrane antigens that are isolated immunospecifically and subjected to limited trypsin digestion followed by polyacrylamide gel electrophoresis in detergent. A large "core" peptide is produced by proteolysis of murine thymus-leukemia antigens (TLA) and from antigens of the major histocompatibility complex (MHC). The tryptic cores from H-2K and H-2D are regularly distinguishable from the thymus-leukemia antigens (TLA) by gel electrophoresis in one dimension. This chemical distinction is particularly important in the analysis of antigen mixtures isolated with antisera specific for beta 2 microglobulin. These techniques have been used to identify thymus-restricted beta 2 microglobulin-associated antigens on cell membranes from mouse, man, guinea pig, and monkey. In appropriate inbred mouse strains, these are the TLA and it is proposed that in the three other species examined they may be analogues, although not necessarily homologues, of TLA. The broad species distribution of these thymus-restricted cell membrane antigens suggests that they are involved in the differentiation of thymus-dependent lymphocytes (T cells).
本文介绍了一种用于鉴定特定细胞膜抗原的简单技术。该方法采用标记的膜抗原，这些抗原通过免疫特异性分离，然后进行有限的胰蛋白酶消化，接着在去污剂中进行聚丙烯酰胺凝胶电泳。通过对鼠胸腺白血病抗原（TLA）和主要组织相容性复合体（MHC）抗原进行蛋白水解可产生一个大的“核心”肽。H-2K和H-2D的胰蛋白酶核心在一维凝胶电泳中通常可与胸腺白血病抗原（TLA）区分开来。这种化学差异在分析用针对β2微球蛋白的抗血清分离的抗原混合物时尤为重要。这些技术已被用于鉴定来自小鼠、人、豚鼠和猴细胞膜上与β2微球蛋白相关的胸腺限制性抗原。在合适的近交小鼠品系中，这些就是TLA，并且有人提出，在所检测的其他三个物种中，它们可能是TLA的类似物，尽管不一定是同源物。这些胸腺限制性细胞膜抗原的广泛物种分布表明它们参与了胸腺依赖性淋巴细胞（T细胞）的分化。
