Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
Talanta. 2012 Jan 30;89:149-54. doi: 10.1016/j.talanta.2011.12.005. Epub 2011 Dec 24.
Successful simultaneous enantioseparation and sensitive determination of three β-blockers (PIN, OX and PRO), have been achieved by capillary electrophoresis using an achiral ionic liquid, [GTMA]Cl, as a modifier to cooperate with dual CDs containing DM-β-CD and TM-β-CD. The influence of aIL was investigated in details, including various aILs, the concentration of aIL and molar ratio of aIL to CD. The ratio of DM-β-CD to TM-β-CD in dual CDs was also discussed. DM-β-CD and TM-β-CD favor the enantioseparations of PIN/OX and PRO, respectively. Meanwhile, the presence of [GTMA]Cl was found to play a key role in enantioseparations, and it widened the scope of application of DM-β-CD and TM-β-CD. Furthermore, FESI as an effective on-line sample enrichment technique was developed to improve the detection sensitivity. Under the optimum conditions, the detection limits of the three pairs of enantiomers range from 0.10 to 0.65 nM, which are much lower than those in the conventional methods. Eventually, the proposed method was successfully applied to the analysis of spiked urine sample with good recoveries.
成功地将三种β受体阻滞剂（PIN、OX 和 PRO）进行对映体拆分和灵敏检测，使用非手性离子液体[GTMA]Cl 作为修饰剂，与含有 DM-β-CD 和 TM-β-CD 的双 CD 配合，通过毛细管电泳实现。详细研究了 aIL 的影响，包括各种 aIL、aIL 的浓度以及 aIL 与 CD 的摩尔比。还讨论了双 CD 中 DM-β-CD 与 TM-β-CD 的比例。DM-β-CD 和 TM-β-CD 分别有利于 PIN/OX 和 PRO 的对映体拆分。同时，发现[GTMA]Cl 的存在对对映体拆分起着关键作用，并拓宽了 DM-β-CD 和 TM-β-CD 的应用范围。此外，开发了 FESI 作为一种有效的在线样品富集技术，以提高检测灵敏度。在最佳条件下，三种对映体对的检测限范围为 0.10 至 0.65 nM，远低于常规方法的检测限。最终，该方法成功应用于加标尿液样品的分析，回收率良好。
