Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
Talanta. 2012 Jan 30;89:280-5. doi: 10.1016/j.talanta.2011.12.029. Epub 2011 Dec 19.
A environment-friendly microwave-assisted extraction used to extract trace mercury compounds from fish samples, and a ultra-sensitive method for the analysis of Hg(II), methylmercury (MeHg) and ethylmercury (EtHg) by using capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) were described in this study. The extraction method is environment-friendly, simple, effective, and can be used to extract trace mercury compounds in fish samples with a satisfied recovery within several minutes. The CE-ICP-MS analytical method has a detection limit as lower as 0.021-0.032 ng Hg/mL for MeHg, EtHg and Hg(II), and can be used to determined ultratrace MeHg, EtHg and Hg(II) in natural water and fish samples directly without any preconcentration. With the help of the above methods, we have successfully determined MeHg, EtHg and Hg(II) in dried fish (Tapertail anchovy) muscle and natural water within 25 min with a RSD (relative standard deviation, n=6) <5% and a recovery of 94-103%. Our results showed that dried muscle of T. anchovy contained only one species of mercury, MeHg, indicating that MeHg is easier to be accumulated by aquatic organisms.
本研究采用一种环境友好型的微波辅助萃取方法从鱼类样品中提取痕量汞化合物，并结合毛细管电泳-电感耦合等离子体质谱（CE-ICP-MS）建立了一种超灵敏分析汞（II）、甲基汞（MeHg）和乙基汞（EtHg）的方法。该萃取方法具有环保、简单、高效的特点，可在数分钟内提取鱼类样品中的痕量汞化合物，且具有较高的回收率。CE-ICP-MS 分析方法对 MeHg、EtHg 和 Hg（II）的检测限低至 0.021-0.032ngHg/mL，可直接用于天然水和鱼类样品中超痕量 MeHg、EtHg 和 Hg（II）的测定，无需任何预浓缩。利用上述方法，我们成功地在 25 分钟内测定了干鱼（小鰶）肌肉和天然水中的 MeHg、EtHg 和 Hg（II），相对标准偏差（n=6）<5%，回收率为 94-103%。结果表明，小鰶肌肉中仅含有一种汞，即 MeHg，表明 MeHg 更容易被水生生物积累。
