Department of Chemistry, Universidad Nacional del Sur, INQUISUR (UNS-CONICET), Av. Alem 1253 (B8000CPB), Bahía Blanca, Buenos Aires, Argentina.
Talanta. 2012 Jan 30;89:526-30. doi: 10.1016/j.talanta.2011.12.019. Epub 2011 Dec 20.
In this study an automatic method for sample treatment and spectrophotometric determination of hydroxyproline in commercial sausages was developed. A flow-batch system that includes the steps of sample hydrolysis and determination of the analyte was designed. The method presents a linear range between 0.60 and 3.60 μg mL(-1) of hydroxyproline. A relative standard deviation of 1.68% was obtained from hydroxyproline standard solution (n=6, 1.20 μg mL(-1)) and the detection limit was 0.12 μg mL(-1). The sample throughput was 1 sample h(-1) while the reference method (AOAC) was carried out in about 17 h. This method employs 16 h of hydrolysis while in the proposed method the hydrolysis time was 15 min. For this purpose a pressure hydrolysis chamber with a low cost halogen lamp was used. The flow-batch system is simple and allows the use of both chambers (hydrolysis and detection) simultaneously. The obtained results with the flow-batch method are in good agreement with that obtained with the reference method. Moreover, it is good alternative to the quality control of meat products.
本研究开发了一种用于商业香肠中羟脯氨酸的自动样品处理和分光光度测定的方法。设计了一种包括样品水解和分析物测定步骤的流动分批系统。该方法在羟脯氨酸标准溶液（n=6，1.20μg/mL）的线性范围为 0.60 至 3.60μg/mL。从羟脯氨酸标准溶液（n=6，1.20μg/mL）中得到的相对标准偏差为 1.68%，检测限为 0.12μg/mL。样品通量为 1 小时/样，而参考方法（AOAC）大约需要 17 小时。该方法采用 16 小时水解，而在提出的方法中，水解时间为 15 分钟。为此，使用了带有低成本卤素灯的压力水解室。流动分批系统简单，允许同时使用两个腔室（水解和检测）。流动分批法获得的结果与参考方法获得的结果吻合良好。此外，它是肉类产品质量控制的良好替代方法。
