Universidade Federal do Piauí, Campus Profa Cinobelina Elvas, Curso de Medicina Veterinária, Bom Jesus, Brazil.
Anim Reprod Sci. 2012 Jan;130(1-2):42-50. doi: 10.1016/j.anireprosci.2011.12.012. Epub 2012 Jan 10.
The objective of the present research was to quantify the seminiferous epithelium cells, spermatogenesis efficiency and characterize the ultrastrucure of Sertoli cells in goats. Eighteen goats were used and divided into three groups: Group I - goats without bipartition of the scrotum; Group II - animals with bipartition of the scrotum in up to 50% of the testicular length; Group III - goats with bipartition of the scrotum in more than 50% of the testicular length. The goat testes in Group III had a greater number of primary spermatocytes (25.37 ± 4.55 cells per cross sections), spermatids (112 ± 15.12 cells per cross sections), and Sertoli cells (9.46 ± 1.74 cells per cross sections) than the animals in Groups I and II (P<0.05). The spermatogenic mitotic, meiotic, and general efficiency were greater in animals in Group III (1.25 ± 0.28; 5.12 ± 1.63; 6.44 ± 1.96) when compared to those in Groups I and II. Sheet-like processes originated from the Sertoli cell body as simple and smooth structures which involved almost all the surface of germ cells. Slender cord-like processes originated from Sertoli cells and also from the sheet-like processes. The relative frequency of the cycle stages showed differences among the groups of goats studied, and the highest frequency was in Stage 3 (20.68% for goats in Group I, 21.15% for those in Group II, and 16.89% for the animals in Group III). In conclusion, goats with bipartition of the scrotum have a greater number of germ and Sertoli cells per cross section of seminiferous tubule, that indicated a greater sperm production when compared to the other groups, and the ultrastructure of the Sertoli cell process did not present any relationship with bipartition of the scrotum.
本研究的目的是量化精子上皮细胞、精子发生效率，并对山羊睾丸支持细胞的超微结构进行特征描述。使用了 18 只山羊，并将其分为三组：第 I 组 - 未对阴囊进行二分法的山羊；第 II 组 - 阴囊二分法长度不超过睾丸长度 50%的动物；第 III 组 - 阴囊二分法长度超过睾丸长度 50%的山羊。第 III 组山羊的初级精母细胞（每个横切面 25.37 ± 4.55 个细胞）、精细胞（每个横切面 112 ± 15.12 个细胞）和支持细胞（每个横切面 9.46 ± 1.74 个细胞）数量多于第 I 和第 II 组（P<0.05）。第 III 组动物的精子发生有丝分裂、减数分裂和总体效率更高（1.25 ± 0.28；5.12 ± 1.63；6.44 ± 1.96），而第 I 和第 II 组则较低。从支持细胞体起源的片状突起呈简单光滑的结构，几乎涉及到所有的生精细胞表面。从支持细胞和片状突起起源的细长索状突起。不同组的山羊中，各周期阶段的相对频率存在差异，第 3 期的频率最高（第 I 组为 20.68%，第 II 组为 21.15%，第 III 组为 16.89%）。总之，阴囊二分法的山羊每个生精小管横切面的生殖细胞和支持细胞数量较多，与其他组相比，精子生成量更大，支持细胞突起的超微结构与阴囊二分法无任何关系。
