Department of Cardiovascular Surgery, Cumhuriyet University School of Medicine, 58140 Sivas, Turkey.
Microvasc Res. 2012 May;83(3):263-6. doi: 10.1016/j.mvr.2012.01.002. Epub 2012 Jan 18.
There is a physiological balance between the stimulatory and inhibitory signals for blood vessel growth. In many symptomatic patients with peripheral artery disease, coronary artery disease, and ischemic chronic wounds, there is a pathological insufficiency of angiogenesis. Therefore, determining the angiogenic or antiangiogenic effects of molecules currently used in cardiovascular treatment is crucial. Although levosimendan is the most well studied calcium sensitizer in preclinical and clinical practice, to the best of our knowledge, there are no previous studies investigating its angiogenic or antiangiogenic effects. In the present study, we aimed to investigate the effects of levosimendan on angiogenesis.
The antiangiogenic efficacy of levosimendan was examined in vivo in the chick chorioallantoic membrane (CAM) model by using 20 fertilized eggs and drug solutions of 1 and 10 μmol/L concentrations. Decreases in the density of the capillaries were assessed and scored.
Significant antiangiogenic effects were observed at 1 and 10 μmol/L concentrations of levosimendan. The antiangiogenic scores of levosimendan at 1 and 10 μmol/L concentrations were 0.6 and 1.10, respectively. The antiangiogenic score of bevacizumab, used as a positive control, was 0.95 at 1.0 μmol/L concentration. No significant difference was found between the antiangiogenic scores of levosimendan and bevacizumab (p=0.54).
Our results indicate that levosimendan has antiangiogenic effects on the chorioallantoic membrane. However, these findings must be confirmed in future studies on humans.
血管生长的刺激和抑制信号之间存在生理平衡。在许多有症状的外周动脉疾病、冠状动脉疾病和缺血性慢性伤口的患者中，存在血管生成的病理性不足。因此，确定目前用于心血管治疗的分子的血管生成或抗血管生成作用至关重要。尽管左西孟旦是临床前和临床实践中研究最充分的钙增敏剂，但据我们所知，以前没有研究调查其血管生成或抗血管生成作用。在本研究中，我们旨在研究左西孟旦对血管生成的影响。
我们在鸡胚绒毛尿囊膜（CAM）模型中用 20 个受精鸡蛋和 1 和 10 μmol/L 浓度的药物溶液来检测左西孟旦的抗血管生成功效。评估并评分毛细血管密度的降低。
在 1 和 10 μmol/L 的左西孟旦浓度下观察到明显的抗血管生成作用。左西孟旦在 1 和 10 μmol/L 浓度下的抗血管生成评分分别为 0.6 和 1.10。作为阳性对照的贝伐单抗在 1.0 μmol/L 浓度下的抗血管生成评分为 0.95。左西孟旦和贝伐单抗的抗血管生成评分之间没有显著差异（p=0.54）。
我们的结果表明，左西孟旦对绒毛尿囊膜具有抗血管生成作用。然而，这些发现必须在未来对人类的研究中得到证实。
