Centre for Bioanalytical Sciences, National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland.
Anal Biochem. 2012 Apr 1;423(1):23-5. doi: 10.1016/j.ab.2011.12.045. Epub 2012 Jan 8.
We developed a method to completely regenerate the gold (Au) surface of 3-aminopropyltriethoxysilane (APTES)-functionalized Au-coated surface plasmon resonance (SPR) chip that had been used for human fetuin A (HFA) immunoassay. It involved treatment of the used SPR chip with freshly prepared piranha solution (concentrated H(2)SO(4)/30% H(2)O(2)=3:1, v/v) for 15 min followed by extensive rinsing with ethanol and deionized water. The developed method enabled prolonged reuse of the regenerated SPR chip that increased its cost-effectiveness without affecting the reproducibility of HFA immunoassays.
我们开发了一种方法，可以完全再生已用于人胎球蛋白 A (HFA) 免疫分析的 3-氨丙基三乙氧基硅烷 (APTES)-功能化 Au 涂覆表面等离子体共振 (SPR) 芯片的 Au 表面。它涉及使用新鲜制备的过硫酸氢钾溶液（浓硫酸/30%双氧水=3:1，v/v）处理用过的 SPR 芯片 15 分钟，然后用乙醇和去离子水充分冲洗。所开发的方法能够延长再生 SPR 芯片的重复使用次数，提高其成本效益，而不会影响 HFA 免疫分析的重现性。
