拟南芥脱水响应元件结合蛋白 2C 的过表达赋予其对氧化胁迫的耐受性。
Overexpression of Arabidopsis dehydration- responsive element-binding protein 2C confers tolerance to oxidative stress.
机构信息
Systems and Synthetic Agrobiotech Center and PMBBRC, Gyeongsang National University, Jinju 660-701, Korea.
出版信息
Mol Cells. 2012 Feb;33(2):135-40. doi: 10.1007/s10059-012-2188-2.
Abstract
Dehydration-responsive element-binding proteins (DREBs)regulate plant responses to environmental stresses. In the current study, transcription of DREB2C, a class 2 Arabidopsis DREB, was induced by a superoxide anion propagator, methyl viologen (MV). The oxidative stress tolerance of DREB2C-overexpressing transgenic plants was significantly greater than that of wild-type plants, as measured by ion leakage and chlorophyll fluorescence under light conditions. The transcriptional activity of several ascorbate peroxidase (APX) genes as well as APX protein activity was induced in DREB2C overexpressors. Additionally, the level of H2O2 in the overexpressors was lower than in wt plants under similar oxidative stress conditions. An electrophoretic mobility shift assay and transient activator reporter assay showed that APX2 expression was regulated by heat shock factor A3 (HsfA3) and that HsfA3 is regulated at the transcriptional level by DREB2C. These results suggest that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis.
摘要
脱水应答元件结合蛋白（DREBs）调控植物对环境胁迫的响应。在本研究中，超氧阴离子引发剂甲紫精（MV）诱导拟南芥 DREB 类 2 型的 DREB2C 转录。通过光条件下的离子渗漏和叶绿素荧光测定，DREB2C 过表达转基因植物的氧化应激耐性显著大于野生型植物。DREB2C 过表达植株中几种抗坏血酸过氧化物酶（APX）基因的转录活性以及 APX 蛋白活性均被诱导。此外，在相似的氧化应激条件下，过表达植株中的 H2O2 水平低于 wt 植物。电泳迁移率变动分析和瞬时激活剂报告分析表明，APX2 的表达受热休克因子 A3（HsfA3）调控，而 HsfA3 则受 DREB2C 在转录水平上的调控。这些结果表明，DREB2C 在促进拟南芥氧化应激耐性方面发挥重要作用。
相似文献
1
Overexpression of Arabidopsis dehydration- responsive element-binding protein 2C confers tolerance to oxidative stress.拟南芥脱水响应元件结合蛋白 2C 的过表达赋予其对氧化胁迫的耐受性。
Mol Cells. 2012 Feb;33(2):135-40. doi: 10.1007/s10059-012-2188-2.
2
Arabidopsis DREB2C functions as a transcriptional activator of HsfA3 during the heat stress response.拟南芥 DREB2C 在热胁迫响应中作为 HsfA3 的转录激活子发挥作用。
Biochem Biophys Res Commun. 2010 Oct 15;401(2):238-44. doi: 10.1016/j.bbrc.2010.09.038. Epub 2010 Sep 16.
3
Overexpression of Heat Shock Factor Gene HsfA3 Increases Galactinol Levels and Oxidative Stress Tolerance in Arabidopsis.热激因子基因HsfA3的过表达增加了拟南芥中肌醇半乳糖苷水平和氧化胁迫耐受性。
Mol Cells. 2016 Jun 30;39(6):477-83. doi: 10.14348/molcells.2016.0027. Epub 2016 Apr 25.
4
Ectopic expression of an Arabidopsis dehydration-responsive element-binding factor DREB2C improves salt stress tolerance in crucifers.拟南芥脱水响应元件结合因子 DREB2C 的异位表达提高十字花科植物的耐盐性。
Plant Cell Rep. 2014 Aug;33(8):1239-54. doi: 10.1007/s00299-014-1612-9. Epub 2014 Apr 16.
5
VOZ1, a transcriptional repressor of DREB2C, mediates heat stress responses in Arabidopsis.VOZ1 作为 DREB2C 的转录抑制子，介导拟南芥的热应激反应。
Planta. 2018 Jun;247(6):1439-1448. doi: 10.1007/s00425-018-2879-9. Epub 2018 Mar 13.
6
Transcriptional regulation of heat shock proteins and ascorbate peroxidase by CtHsfA2b from African bermudagrass conferring heat tolerance in Arabidopsis.非洲狗牙根 CtHsfA2b 通过调控热激蛋白和抗坏血酸过氧化物酶的转录水平赋予拟南芥耐热性。
Sci Rep. 2016 Jun 20;6:28021. doi: 10.1038/srep28021.
7
A proximal promoter region of Arabidopsis DREB2C confers tissue-specific expression under heat stress.拟南芥 DREB2C 的一个近端启动子区域在热胁迫下赋予组织特异性表达。
J Integr Plant Biol. 2012 Sep;54(9):640-51. doi: 10.1111/j.1744-7909.2012.01137.x. Epub 2012 Aug 30.
8
A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis.转录因子DREB2A和热应激转录因子HsfA3的级联反应调控拟南芥的热应激反应。
Plant J. 2008 Jan;53(2):264-74. doi: 10.1111/j.1365-313X.2007.03334.x. Epub 2007 Nov 12.
9
The 26S proteasome function and Hsp90 activity involved in the regulation of HsfA2 expression in response to oxidative stress.26S 蛋白酶体的功能和 Hsp90 活性参与了氧化应激对 HsfA2 表达的调节。
Plant Cell Physiol. 2010 Mar;51(3):486-96. doi: 10.1093/pcp/pcq015. Epub 2010 Feb 9.
10
Heat stress- and heat shock transcription factor-dependent expression and activity of ascorbate peroxidase in Arabidopsis.拟南芥中热应激和热激转录因子依赖性抗坏血酸过氧化物酶的表达与活性
Plant Physiol. 2002 Jun;129(2):838-53. doi: 10.1104/pp.001362.
引用本文的文献
1
Genome-Wide Identification and Comprehensive Analysis of Gene Family in Under Abiotic Stress.非生物胁迫下基因家族的全基因组鉴定与综合分析
Life (Basel). 2025 Aug 11;15(8):1269. doi: 10.3390/life15081269.
2
Identification of DREB gene family in foxtail millet () and analysis of its expression pattern in response to abiotic stress.谷子DREB基因家族的鉴定及其对非生物胁迫响应的表达模式分析。
Front Plant Sci. 2025 Apr 28;16:1552120. doi: 10.3389/fpls.2025.1552120. eCollection 2025.
3
Plant Signaling Hormones and Transcription Factors: Key Regulators of Plant Responses to Growth, Development, and Stress.植物信号激素与转录因子：植物生长、发育及胁迫响应的关键调控因子
Plants (Basel). 2025 Mar 31;14(7):1070. doi: 10.3390/plants14071070.
4
Integrating gene expression analysis and ecophysiological responses to water deficit in leaves of tomato plants.整合基因表达分析和番茄叶片水分亏缺的生理生态响应。
Sci Rep. 2024 Nov 22;14(1):29024. doi: 10.1038/s41598-024-80261-0.
5
and Genes in Garlic ( L.): Genome-Wide Identification, Characterization, and Stress Response.大蒜（L.）中的基因：全基因组鉴定、表征及应激反应
Plants (Basel). 2023 Jul 3;12(13):2538. doi: 10.3390/plants12132538.
6
Genome-wide identification and expression analysis of DREB family genes in cotton.棉花 DREB 家族基因的全基因组鉴定和表达分析。
BMC Plant Biol. 2023 Mar 30;23(1):169. doi: 10.1186/s12870-023-04180-4.
7
Genome-wide identification, comprehensive characterization of transcription factors, cis-regulatory elements, protein homology, and protein interaction network of DREB gene family in .某植物中DREB基因家族的全基因组鉴定、转录因子、顺式调控元件、蛋白质同源性及蛋白质相互作用网络的全面表征
Front Plant Sci. 2022 Nov 24;13:1031679. doi: 10.3389/fpls.2022.1031679. eCollection 2022.
8
Improved abiotic stress tolerance in Arabidopsis by constitutive active form of a banana DREB2 type transcription factor, MaDREB20.CA, than its native form, MaDREB20.通过组成型激活的香蕉 DREB2 类型转录因子 MaDREB20.CA 提高拟南芥的非生物胁迫耐受性，优于其天然形式 MaDREB20。
Protoplasma. 2023 May;260(3):671-690. doi: 10.1007/s00709-022-01805-7. Epub 2022 Aug 23.
9
Elucidating the genetic architecture controlling antioxidant status and ionic balance in barley under salt stress.解析盐胁迫下控制大麦抗氧化状态和离子平衡的遗传结构。
Plant Mol Biol. 2022 Oct;110(3):287-300. doi: 10.1007/s11103-022-01302-8. Epub 2022 Aug 2.
10
Over-Expression of Dehydroascorbate Reductase Improves Salt Tolerance, Environmental Adaptability and Productivity in .脱氢抗坏血酸还原酶的过表达提高了……的耐盐性、环境适应性和生产力。 （原文句末不完整，缺少具体对象）
Antioxidants (Basel). 2022 May 28;11(6):1077. doi: 10.3390/antiox11061077.
本文引用的文献
1
Arabidopsis DREB2C functions as a transcriptional activator of HsfA3 during the heat stress response.拟南芥 DREB2C 在热胁迫响应中作为 HsfA3 的转录激活子发挥作用。
Biochem Biophys Res Commun. 2010 Oct 15;401(2):238-44. doi: 10.1016/j.bbrc.2010.09.038. Epub 2010 Sep 16.
2
DREB2C interacts with ABF2, a bZIP protein regulating abscisic acid-responsive gene expression, and its overexpression affects abscisic acid sensitivity.DREB2C 与 ABF2 相互作用，ABF2 是一种 bZIP 蛋白，调节脱落酸响应基因的表达，其过表达影响脱落酸的敏感性。
Plant Physiol. 2010 Jun;153(2):716-27. doi: 10.1104/pp.110.154617. Epub 2010 Apr 15.
3
Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes.综合分析编码参与非生物胁迫应答基因表达的转录因子的水稻 DREB2 型基因。
Mol Genet Genomics. 2010 Feb;283(2):185-96. doi: 10.1007/s00438-009-0506-y. Epub 2010 Jan 5.
4
Identification of potential DREB2C targets in Arabidopsis thaliana plants overexpressing DREB2C using proteomic analysis.利用蛋白质组学分析鉴定拟南芥中过表达 DREB2C 植物的潜在 DREB2C 靶标。
Mol Cells. 2009 Oct 31;28(4):383-8. doi: 10.1007/s10059-009-0154-4. Epub 2009 Oct 13.
5
The HaDREB2 transcription factor enhances basal thermotolerance and longevity of seeds through functional interaction with HaHSFA9.HaDREB2转录因子通过与HaHSFA9的功能相互作用增强种子的基础耐热性和寿命。
BMC Plant Biol. 2009 Jun 19;9:75. doi: 10.1186/1471-2229-9-75.
6
Functional analysis of an Arabidopsis heat-shock transcription factor HsfA3 in the transcriptional cascade downstream of the DREB2A stress-regulatory system.拟南芥热激转录因子HsfA3在DREB2A胁迫调控系统下游转录级联反应中的功能分析
Biochem Biophys Res Commun. 2008 Apr 11;368(3):515-21. doi: 10.1016/j.bbrc.2008.01.134. Epub 2008 Feb 7.
7
Generation of superoxide anion in chloroplasts of Arabidopsis thaliana during active photosynthesis: a focus on rapidly induced genes.拟南芥叶绿体在活跃光合作用过程中超氧阴离子的产生：聚焦快速诱导基因。
Plant Mol Biol. 2008 Mar;66(4):361-78. doi: 10.1007/s11103-007-9274-4. Epub 2007 Dec 25.
8
A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis.转录因子DREB2A和热应激转录因子HsfA3的级联反应调控拟南芥的热应激反应。
Plant J. 2008 Jan;53(2):264-74. doi: 10.1111/j.1365-313X.2007.03334.x. Epub 2007 Nov 12.
9
Identification of a calmodulin-binding NAC protein as a transcriptional repressor in Arabidopsis.鉴定一种钙调蛋白结合NAC蛋白作为拟南芥中的转录抑制因子。
J Biol Chem. 2007 Dec 14;282(50):36292-302. doi: 10.1074/jbc.M705217200. Epub 2007 Oct 18.
10
Arabidopsis thaliana deficient in two chloroplast ascorbate peroxidases shows accelerated light-induced necrosis when levels of cellular ascorbate are low.当细胞内抗坏血酸水平较低时，缺乏两种叶绿体抗坏血酸过氧化物酶的拟南芥会出现光诱导坏死加速的现象。
Plant Mol Biol. 2007 Nov;65(5):627-44. doi: 10.1007/s11103-007-9227-y. Epub 2007 Sep 7.
Zotero 插件