Riljak Vladimír, Benes Jan, Pokorný Jaroslav, Myslivecek Jaromir
Institute of Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
Neuro Endocrinol Lett. 2011;32(6):816-20.
Our previous study showed that administration of nicotine is capable to protect the neurons of hippocampus against the kainic acid induced damage. Here we tested the hypotheses that multiple nicotine administration would prevent the effects of kainic acid on neuronal nicotinic receptor subtypes densities (α-bungarotoxin sensitive and α-bungarotoxin insentive) and on hippocampal cell degeneration.
Radioligand binding study was used to detect the particular nAChR subtypes densities. Two histochemical methods (bis-benzimide staining and Fluoro-Jade B dye) were used to detect and evaluate neuronal degeneration.
Our study shows that: a) kainic acid single administration increased the number of α-bungarotoxin insentive nicotinic receptors, b) nicotine was able to prevent such changes, c) repeated nicotine administration is capable to attenuate the damage of CA1 and CA3 areas of the hippocampus. No effect on α-bungarotoxin sentive nicotinic receptors was observed. Our data therefore reveal the importance of α-bungarotoxin insentive nicotinic receptors in the response to kainite and the ability of nicotine to prevent such changes both in the cell degeneration and in number of receptors.
Nicotine administration influences α-bungarotoxin insensitive receptors and repeated administration is capable to protect against toxicity caused by kainic acid in hippocampal area.
我们之前的研究表明，给予尼古丁能够保护海马神经元免受海藻酸诱导的损伤。在此，我们检验了以下假设：多次给予尼古丁可预防海藻酸对神经元烟碱受体亚型密度（α-银环蛇毒素敏感型和α-银环蛇毒素不敏感型）以及海马细胞变性的影响。
采用放射性配体结合研究来检测特定的烟碱型乙酰胆碱受体（nAChR）亚型密度。使用两种组织化学方法（双苯甲酰胺染色和荧光金B染料）来检测和评估神经元变性。
我们的研究表明：a）单次给予海藻酸会增加α-银环蛇毒素不敏感型烟碱受体的数量，b）尼古丁能够预防此类变化，c）重复给予尼古丁能够减轻海马CA1和CA3区域的损伤。未观察到对α-银环蛇毒素敏感型烟碱受体有影响。因此，我们的数据揭示了α-银环蛇毒素不敏感型烟碱受体在对海藻酸盐反应中的重要性，以及尼古丁预防细胞变性和受体数量变化的能力。
给予尼古丁会影响α-银环蛇毒素不敏感型受体，重复给予能够预防海马区海藻酸引起的毒性。
