Matsukuma S, Durston A J
J Embryol Exp Morphol. 1979 Apr;50:243-51.
We made mixed mounds of vitally stained prestalk cells and unstained prespore cells, from Dictyostelium discoideum (Dd), and exposed these to external 3',5'-cyclic-AMP (c-AMP) sources. We show that prestalk cells will sort out by moving directionally toward c-AMP sources held at less than 10(-5) M c-AMP. This is evidence for cell sorting via chemotaxis. We present arguments that the natural cell sorting, observed during Dd development, is also chemotactic to c-AMP.
我们从盘基网柄菌（Dd）中制备了经活体染色的前柄细胞和未染色的前孢子细胞的混合细胞团，并将这些细胞团暴露于外部的3'，5'-环磷酸腺苷（c-AMP）源。我们发现，前柄细胞会通过向浓度低于10^(-5) M的c-AMP源定向移动来进行分选。这是通过趋化作用进行细胞分选的证据。我们提出论据表明，在Dd发育过程中观察到的自然细胞分选对c-AMP也具有趋化性。
