Department of Biology, Syracuse University, Syracuse, NY, USA.
J Bacteriol. 2012 Apr;194(7):1689-96. doi: 10.1128/JB.06492-11. Epub 2012 Jan 27.
The Clp/Hsp100 proteins are chaperones that play a role in protein degradation and reactivation. In bacteria, they exhibit a high degree of pleiotropy, affecting both individual and multicellular phenotypes. In this article, we present the first characterization of a Clp/Hsp100 homolog in Myxococcus xanthus (MXAN_4832 gene locus). Deletion of MXAN_4832 causes defects in both swarming and aggregation related to cell motility and the production of fibrils, which are an important component of the extracellular matrix of a swarm. The deletion also affects the formation of myxospores during development, causing them to become sensitive to heat. The protein product of MXAN_4832 can act as a chaperone in vitro, providing biochemical evidence in support of our hypothesis that MXAN_4832 is a functional Clp/Hsp100 homolog. There are a total of 12 Clp/Hsp100 homologs in M. xanthus, including MXAN_4832, and, based on its mutational and biochemical characterization, they may well represent an important group.
Clp/Hsp100 蛋白是一类在蛋白质降解和重激活过程中发挥作用的伴侣蛋白。在细菌中，它们表现出高度的多效性，影响个体和多细胞表型。在本文中，我们首次对粘细菌（Myxococcus xanthus）中的 Clp/Hsp100 同源物（MXAN_4832 基因座）进行了描述。MXAN_4832 的缺失导致游动和聚集相关的缺陷，这与细胞运动和纤丝的产生有关，纤丝是游动体细胞外基质的重要组成部分。该缺失还影响发育过程中粘孢子的形成，导致它们对热敏感。MXAN_4832 的蛋白产物在体外可以作为伴侣蛋白发挥作用，为我们的假设提供了生化证据，即 MXAN_4832 是一种功能性的 Clp/Hsp100 同源物。粘细菌共有 12 种 Clp/Hsp100 同源物，包括 MXAN_4832，根据其突变和生化特征，它们可能代表了一个重要的群体。
