异-15:0 O-烷基甘油部分是黄色黏球菌中E信号的关键结构。
An iso-15 : 0 O-alkylglycerol moiety is the key structure of the E-signal in Myxococcus xanthus.
作者信息
Ahrendt Tilman, Dauth Christina, Bode Helge B
机构信息
Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main, Germany.
Buchmann Institute for Molecular Life Sciences (BMLS), Goethe Universität Frankfurt, Max-von-Laue-Strasse 15, 60438 Frankfurt am Main, Germany.
出版信息
Microbiology (Reading). 2016 Jan;162(1):138-144. doi: 10.1099/mic.0.000169. Epub 2015 Sep 4.
The E-signal is one of five intercellular signals (named A- to E-signal) guiding fruiting body development in Myxococcus xanthus, and it has been shown to be a combination of the branched-chain fatty acid (FA) iso-15 : 0 and the diacylmonoalkyl ether lipid TG1. Developmental mutants HB015 (Δbkd MXAN_4265::kan) and elbD (MXAN_1528::kan) are blocked at different stages of fruiting body and spore formation as they cannot form the required iso-FA or the actual ether lipid, respectively. In order to define the structural basis of the E-signal, different mono- and triglycerides containing ether or ester bonds were synthesized and used for complementation of these mutants. Here, the monoalkylglyceride dl-1-O-(13-methyltetradecyl)glycerol exhibited comparably high levels of complementation in both mutants, restoring fruiting body and spore formation, identifying iso-15 : 0 O-alkylglycerol, part of the natural lipid TG1, as the 'signalophore' of E-signalling.
E信号是引导黄色粘球菌子实体发育的五种细胞间信号之一（命名为A至E信号），并且已证明它是支链脂肪酸（FA）异-15:0和二酰基单烷基醚脂质TG1的组合。发育突变体HB015（Δbkd MXAN_4265::kan）和elbD（MXAN_1528::kan）分别在子实体和孢子形成的不同阶段受阻，因为它们不能形成所需的异脂肪酸或实际的醚脂质。为了确定E信号的结构基础，合成了含有醚键或酯键的不同甘油单酯和甘油三酯，并用于这些突变体的互补实验。在此，单烷基甘油dl-1-O-(13-甲基十四烷基)甘油在两个突变体中均表现出相当高的互补水平，恢复了子实体和孢子形成，确定异-15:0 O-烷基甘油（天然脂质TG1的一部分）为E信号传导的“信号团”。
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