Beale K M, Kunkle G A, Chalker L, Cannon R
Blanche Saunders Dermatology Laboratory, College of Veterinary Medicine, University of Florida, Gainesville 32610-0126.
J Am Vet Med Assoc. 1990 Oct 1;197(7):861-4.
Effects of 4 commonly used sedatives on the wheal-and-flare response to histamine and flea antigen were evaluated in 8 flea-allergic Beagles. Skin testing was performed in 12 separate occasions, 3 to 4 days apart. Twelve intradermal injections were given during each skin test: 5 doubling dilutions of histamine phosphate, 6 doubling dilutions of flea antigen, and a phosphate-buffered saline solution (negative control). Of the 12 intradermal skin tests, 8 were control tests performed on nonsedated dogs. The remaining 4 tests were performed on dogs sedated with xylazine, ketamine and valium combination, acepromazine, or oxymorphone. Oxymorphone had the most profound effect on skin test results, significantly (P less than 0.05) decreasing skin responsiveness in 8 of 11 test sites (by objective evaluation) and in 5 of 11 test sites (by subjective evaluation). Xylazine sedation enhanced skin test results in 4 of 11 test sites (by objective evaluation) and in 1 of 11 test sites (by subjective evaluation). In non instance did xylazine significantly decrease skin responsiveness to histamine or flea antigen. Xylazine is the recommended sedative in dogs when sedation is necessary for intradermal skin testing.
在8只对跳蚤过敏的比格犬中评估了4种常用镇静剂对组胺和跳蚤抗原所致风团-潮红反应的影响。皮肤试验在12个不同时间进行，间隔3至4天。每次皮肤试验时进行12次皮内注射：5种磷酸组胺的倍比稀释液、6种跳蚤抗原的倍比稀释液以及一种磷酸盐缓冲盐水溶液（阴性对照）。在这12次皮内皮肤试验中，8次是对未镇静的犬进行的对照试验。其余4次试验是对用赛拉嗪、氯胺酮和安定组合、乙酰丙嗪或羟吗啡酮镇静的犬进行的。羟吗啡酮对皮肤试验结果影响最为显著，在11个试验部位中的8个（通过客观评估）和11个试验部位中的5个（通过主观评估）显著（P<0.05）降低皮肤反应性。赛拉嗪镇静在11个试验部位中的4个（通过客观评估）和11个试验部位中的1个（通过主观评估）增强了皮肤试验结果。在任何情况下赛拉嗪都未显著降低皮肤对组胺或跳蚤抗原的反应性。当皮内皮肤试验需要镇静时，赛拉嗪是犬推荐使用的镇静剂。
