Kristensen S, Kieffer M
Nord Vet Med. 1978 Oct;30(10):414-23.
A group of 143 dogs and cats with pruritic skin disease was tested by intradermal injection of a dilute whole cat flea extract. The test results were correlated with the clinical diagnosis (Table I, III), with previous or present findings of fleas on the animals (Table II, III), with the presence or absence of blood eosinophilia (Table IV), and, in animals with clinical flea allergy, with the histological findings in a skin biopsy excised from a clinically affected skin site (Fig. 1). The predictive value of a positive and a negative outcome of the test was assessed graphically by means of calculations described in the Appendix (Figs. 2, 3). It is concluded that the test is sufficiently specific to justify its use in animals where flea allergy is suspected but cannot be proved by routine clinical examination. The histological findings and the nature of the skin reactions suggest that a positive outcome of the test is indicative of an immediate, type I hypersensitivity towards components of the oral secretion of fleas and that this hypersensitivity is mediated by reaginic antibody which to some extent is produced by plasma cells located in skin areas where flea saliva is deposited.
一组143只患有瘙痒性皮肤病的犬猫通过皮内注射稀释的全猫蚤提取物进行检测。将检测结果与临床诊断（表I、III）、动物身上先前或当前的跳蚤发现情况（表II、III）、血液嗜酸性粒细胞增多症的有无（表IV）相关联，对于患有临床跳蚤过敏的动物，还与从临床受影响皮肤部位切除的皮肤活检组织学结果（图1）相关联。通过附录中描述的计算方法（图2、3）以图形方式评估检测阳性和阴性结果的预测价值。得出的结论是，该检测具有足够的特异性，证明其可用于疑似跳蚤过敏但常规临床检查无法证实的动物。组织学结果和皮肤反应的性质表明，检测阳性结果表明对跳蚤口腔分泌物成分存在即刻I型超敏反应，并且这种超敏反应由反应素抗体介导，该抗体在一定程度上由位于跳蚤唾液沉积皮肤区域的浆细胞产生。
