Cytotoxicity of hydrophylic silver carboxylato complexes.

BACKGROUND

Gold(I) and platinum(II) d(10) and d(8) electronic complexes such as (Au(PPh(2)CH(2)CH(2)PPh(2))(2))Cl and cisplatin, ((H(3)N)(2)PtCl(2)), possess strong antineoplastic activities. Almost no information is available regarding the anticancer activity of isoelectronic silver(I) d(10) complexes. This study examined the cytotoxicity of stable water-soluble silver(I) carboxylates. The results were related to the cytotoxicity of cisplatin and (Au(PPh(2)CH(2)CH(2)PPh(2))(2))Cl.

MATERIALS AND METHODS

The silver carboxylates (AgO(2)CCH(2)OCH(3)), 1, (AgO(2)C-CH(2)OCH(2)CH(2)OCH(3)), 2, and (AgO(2)CCH(2)OCH(2)CH(2)OCH(2)CH(2)OCH(3)), 3, were investigated. Cytotoxicity tests were performed on the HeLa (human cervix epitheloid) cancer cell line, resting lymphocytes and PHA (phytohaemagglutinin)-stimulated lymphocyte cultures. Cell survival was measured by means of the colorometric 3-(4,5-dimethylthiazol-2-yl)-diphenyltetrazolium bromide (MTT) assay.

RESULTS

The IC(50) (50% cell growth inhibition) values of 1-3 in the HeLa cells varied between 2.6 and 6.1 μmol dm(-3) with being 1 the most cytotoxic silver complex. Drug activity was inversely proportional to the length of the carboxylato chain length. Complexes 1-3 were 3-5 times more cytotoxic against the HeLa cancer cells than against the PHA stimulated lymphocyte cultures.

CONCLUSION

A drug activity-structure relationship exists in that short-chain silver carboxylates are more cytotoxic than long-chain silver carboxylates, but silver d(10) complexes are one order of magnitude less cytotoxic than platinum(II) d(8) or gold(I) d(10) complexes.