Grefte Sander, Kuijpers Mette A R, Kuijpers-Jagtman Anne M, Torensma Ruurd, Von den Hoff Johannes W
Radboud University Nijmegen Medical Centre, Department of Orthodontics and Craniofacial Biology, Nijmegen, The Netherlands.
Eur J Oral Sci. 2012 Feb;120(1):38-45. doi: 10.1111/j.1600-0722.2011.00920.x. Epub 2012 Jan 23.
The restoration of muscles in the soft palate of patients with cleft lip and/or palate is accompanied by fibrosis, which leads to speech and feeding problems. Treatment strategies that improve muscle regeneration have only been tested in limb muscles. Therefore, in the present study the myogenic potential of muscle progenitor cells (MPCs) isolated from head muscles was compared with that of limb muscles. Muscle progenitor cells were isolated from the head muscles and limb muscles of rats and cultured. The proliferation of MPCs was analysed by DNA quantification. The differentiation capacity was analysed by quantifying the numbers of fused cells, and by measuring the mRNA levels of differentiation markers. Muscle progenitor cells were stained to quantify the expression of paired box protein Pax 7 (Pax-7), myoblast determination protein 1 (MyoD), and myogenin. Proliferation was similar in the head MPCs and the limb MPCs. Differentiating head and limb MPCs showed a comparable number of fused cells and mRNA expression levels of myosin-1 (Myh1), myosin-3 (Myh3), and myosin-4 (Myh4). During proliferation and differentiation, the number of Pax-7(+), MyoD(+), and myogenin(+) cells in head and limb MPCs was equal. It was concluded that head and limb MPCs show similar myogenic capacities in vitro. Therefore, in vivo myogenic differences between those muscles might rely on the local microenvironment. Thus, regenerative strategies for limb muscles might also be used for head muscles.
唇裂和/或腭裂患者软腭肌肉的修复伴随着纤维化，这会导致言语和进食问题。改善肌肉再生的治疗策略仅在肢体肌肉中进行过测试。因此，在本研究中，将从头部肌肉分离的肌肉祖细胞（MPCs）与肢体肌肉的肌肉祖细胞的生肌潜能进行了比较。从大鼠的头部肌肉和肢体肌肉中分离出肌肉祖细胞并进行培养。通过DNA定量分析MPCs的增殖情况。通过量化融合细胞的数量以及测量分化标志物的mRNA水平来分析分化能力。对肌肉祖细胞进行染色以量化配对盒蛋白Pax 7（Pax-7）、成肌细胞决定蛋白1（MyoD）和肌细胞生成素的表达。头部MPCs和肢体MPCs的增殖情况相似。正在分化的头部和肢体MPCs显示出相当数量的融合细胞以及肌球蛋白-1（Myh1）、肌球蛋白-3（Myh3）和肌球蛋白-4（Myh4）的mRNA表达水平。在增殖和分化过程中，头部和肢体MPCs中Pax-7(+)、MyoD(+)和肌细胞生成素(+)细胞的数量相等。得出的结论是，头部和肢体MPCs在体外显示出相似的生肌能力。因此，这些肌肉之间的体内生肌差异可能取决于局部微环境。因此，肢体肌肉的再生策略也可能用于头部肌肉。
