Chen X, Qin Z
Department of Otolaryngology, Institute of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, 1 JianShe East Road, ZhengZhou, Henan 450052, China.
J Int Med Res. 2011;39(6):2110-8. doi: 10.1177/147323001103900607.
This study investigated the role of microRNA-21 (miR-21) and let-7a microRNA in paediatric and adult cholesteatoma. Total RNA and protein were isolated from the cholesteatoma specimens and normal skin of 10 adults and 10 children. Levels of miR-21 and let-7a microRNA were assessed by real-time reverse transcription-polymerase chain reaction, and levels of phosphatase and tensin homologue (PTEN), programmed cell death 4 (PDCD4) and high mobility group AT-hook 2 (HMGA2) protein were assessed by Western blot analysis. Levels of miR-21 and let-7a microRNA were significantly higher in cholesteatoma tissue compared with normal skin, especially in paediatric patients. PTEN, PDCD4 and HMGA2 protein levels were significantly lower in paediatric versus adult cholesteatoma patients. It is possible that upregulation of miR-21 leads to higher tumour cell proliferation and invasion of cholesteatoma in children than adults, and the benign nature of cholesteatoma may be due to a balance between let-7a microRNA and miR-21. These data may help to identify targets for miRNA- and protein-based therapeutic interventions for the non-surgical or adjunctive treatment of cholesteatoma.
本研究调查了微小RNA-21（miR-21）和微小RNA let-7a在儿童及成人胆脂瘤中的作用。从10名成人和10名儿童的胆脂瘤标本及正常皮肤中分离出总RNA和蛋白质。通过实时逆转录-聚合酶链反应评估miR-21和let-7a微小RNA的水平，通过蛋白质免疫印迹分析评估磷酸酶和张力蛋白同源物（PTEN）、程序性细胞死亡4（PDCD4）和高迁移率族AT钩蛋白2（HMGA2）的蛋白质水平。与正常皮肤相比，胆脂瘤组织中miR-21和let-7a微小RNA的水平显著更高，尤其是在儿科患者中。儿科胆脂瘤患者的PTEN、PDCD4和HMGA2蛋白质水平显著低于成人。miR-21的上调可能导致儿童胆脂瘤的肿瘤细胞增殖和侵袭高于成人，胆脂瘤的良性性质可能归因于let-7a微小RNA和miR-21之间的平衡。这些数据可能有助于确定基于微小RNA和蛋白质的治疗干预靶点，用于胆脂瘤的非手术或辅助治疗。
