Sugai M, Akiyama T, Komatsuzawa H, Miyake Y, Suginaka H
Department of Microbiology, Hiroshima University School of Dentistry, Japan.
J Bacteriol. 1990 Nov;172(11):6494-8. doi: 10.1128/jb.172.11.6494-6498.1990.
Profiles of the bacteriolytic activities of Staphylococcus aureus culture supernatants, sodium dodecyl sulfate cell extracts, LiCl cell extracts, cell wall extracts, and cell membranes were analyzed in sodium dodecyl sulfate-polyacrylamide gels containing Micrococcus luteus or S. aureus. A total of 20 distinct bands of bacteriolytic activity could be detected in gels containing M. luteus, 8 of these bands were found in culture supernatants. The sodium dodecyl sulfate cell extracts, the LiCl cell extracts, and the cell membranes each contained 20 bands (P1 to P20), but no activity was found in cell wall extracts. Less bacteriolytic activity could be detected in gels containing S. aureus, although three bands were found in culture supernatants and LiCl extracts and cell membranes contained one major band, P13. Crude cell extracts showed five bacteriolytic bands of which the major bacteriolytic bands were distributed in an identical manner in all 10 strains of S. aureus studied. The effects of chemical and physical factors were determined, and it was shown that iodoacetic acid, Hg2+, and Cibacron Blue 3G-A reduced activity, and an optimum pH for enzyme detection was between 7 and 8. Preincubation at 100 degrees C for 30 min reduced the activity of P1 and P2 bands.
在含有藤黄微球菌或金黄色葡萄球菌的十二烷基硫酸钠-聚丙烯酰胺凝胶中，分析了金黄色葡萄球菌培养上清液、十二烷基硫酸钠细胞提取物、氯化锂细胞提取物、细胞壁提取物和细胞膜的溶菌活性谱。在含有藤黄微球菌的凝胶中总共可检测到20条不同的溶菌活性带，其中8条带存在于培养上清液中。十二烷基硫酸钠细胞提取物、氯化锂细胞提取物和细胞膜各自含有20条带（P1至P20），但在细胞壁提取物中未发现活性。在含有金黄色葡萄球菌的凝胶中可检测到的溶菌活性较少，尽管在培养上清液中发现了三条带，并且氯化锂提取物和细胞膜含有一条主要带P13。粗细胞提取物显示出五条溶菌带，其中主要溶菌带在所有研究的10株金黄色葡萄球菌中以相同方式分布。测定了化学和物理因素的影响，结果表明碘乙酸、Hg2+和汽巴蓝3G-A降低了活性，酶检测的最佳pH在7至8之间。在100℃预孵育30分钟降低了P1和P2带的活性。
