van Doormaal J J, Tijhuis G J, Zwertbroek R, Muskiet F A, Velvis H J, Smit Sibinga C T, Doorenbos H
Department of Endocrinology, University Hospital, Groningen, The Netherlands.
J Clin Apher. 1990;5(4):179-82. doi: 10.1002/jca.2920050402.
The effect of a rapid reduction of plasma lipoproteins on the alpha- and gamma-tocopherol levels in plasma, erythrocytes, and platelets was studied. Sixteen successive plasma exchange procedures performed weekly in an adult with heterozygous familial hypercholesterolemia were evaluated. Plasma exchange was done by intermittent flow centrifugation, exchanging one plasma volume against a 4% human albumin solution. Plasma exchange reduced in plasma alpha-tocopherol from 41.5 +/- 8.9 to 23.6 +/- 4.8 mumol/L and gamma-tocopherol from 4.9 +/- 4.1 to 2.4 +/- 2.1 mumol/L, without changing their ratios to total lipids. It diminished alpha-tocopherol in platelets from 12.97 +/- 4.37 to 10.03 +/- 1.78 mumol/10(13) cells and gamma-tocopherol from 1.43 +/- 0.55 to 1.06 +/- 0.41 mumol/10(13) cells, but did not affect erythrocyte tocopherols. The total amount removed per procedure was 47.57 +/- 13.65 mumol for alpha-tocopherol and 4.70 +/- 3.59 mumol for gamma-tocopherol. Plasma exchange increased the number of erythrocytes from 3.67 +/- 0.10.10(12) to 4.05 +/- 0.13.10(12) cells/L, without affecting their volume. Platelet count did not change, but mean platelet volume decreased from 7.7 +/- 0.5 to 6.9 +/- 0.5 fl and platelet distribution width from 15.1 +/- 0.4 to 14.9 +/- 0.5. Thus, plasma exchange reduces plasma alpha- and gamma-tocopherol to the same extent as total lipids, and decreases these tocopherols in circulating platelets, along with a reduction in platelet size and, compared to the change in erythrocyte count, a fall of platelet number.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了血浆脂蛋白快速降低对血浆、红细胞和血小板中α-生育酚和γ-生育酚水平的影响。对一名患有杂合子家族性高胆固醇血症的成年人每周进行16次连续的血浆置换程序进行了评估。血浆置换通过间歇流动离心法进行，用4%的人白蛋白溶液置换一个血浆体积。血浆置换使血浆中α-生育酚从41.5±8.9μmol/L降至23.6±4.8μmol/L，γ-生育酚从4.9±4.1μmol/L降至2.4±2.1μmol/L，而它们与总脂质的比率不变。它使血小板中的α-生育酚从12.97±4.37μmol/10¹³细胞降至10.03±1.78μmol/10¹³细胞，γ-生育酚从1.43±0.55μmol/10¹³细胞降至1.06±0.41μmol/10¹³细胞，但不影响红细胞中的生育酚。每次程序去除的总量，α-生育酚为47.57±13.65μmol，γ-生育酚为4.70±3.59μmol。血浆置换使红细胞数量从3.67±0.10×10¹²/L增加到4.05±0.13×10¹²/L，而不影响其体积。血小板计数未改变，但平均血小板体积从7.7±0.5fl降至6.9±0.5fl，血小板分布宽度从15.1±0.4降至14.9±0.5。因此，血浆置换使血浆α-生育酚和γ-生育酚与总脂质降低到相同程度，并降低循环血小板中的这些生育酚，同时血小板大小减小，与红细胞计数的变化相比，血小板数量减少。（摘要截于250字）
