Oyama R, Suzuki M, Matsuzawa T, Titani K
Division of Biomedical Polymer Science, Fujita Health University, Aichi.
J Biochem. 1990 Jul;108(1):133-8. doi: 10.1093/oxfordjournals.jbchem.a123152.
The complete amino acid sequence of rat kidney ornithine aminotransferase [EC 2.6.1.13] is presented. The 404-residue sequence was determined by analysis of peptides generated by digestion of the S-carboxyamidomethylated protein with CNBr, Achromobacter protease I, arginylendopeptidase, or Staphylococcus aureus V8 protease. Mueckler and Pitot have reported the amino acid sequence of the rat liver enzyme (440 residues) as predicted from the nucleotide sequence of the cDNA [Mueckler, M.M. & Pitot, H.C. (1985) J. Biol. Chem. 260, 12993-12997]. The amino acid sequence of the rat kidney enzyme presented herein coincides with residue 36 (Gly) through 440 (Phe) of the predicted precursor protein, indicating that the liver and kidney enzymes are identical, and that the enzyme is processed at the amino-terminal region after translation.
本文给出了大鼠肾脏鸟氨酸氨基转移酶[EC 2.6.1.13]的完整氨基酸序列。通过分析用溴化氰、无色杆菌蛋白酶I、精氨酰内肽酶或金黄色葡萄球菌V8蛋白酶消化S-羧甲基化蛋白产生的肽段，确定了这一包含404个残基的序列。Mueckler和Pitot已报道了根据cDNA核苷酸序列预测的大鼠肝脏酶的氨基酸序列（440个残基）[Mueckler, M.M. & Pitot, H.C. (1985) J. Biol. Chem. 260, 12993 - 12997]。本文给出的大鼠肾脏酶的氨基酸序列与预测的前体蛋白的第36位残基（甘氨酸）至440位残基（苯丙氨酸）一致，这表明肝脏和肾脏中的酶是相同的，且该酶在翻译后于氨基末端区域进行了加工。
