Traoré Amadou, Royo Luis J, Kaboré Adama, Pérez-Pardal Lucía, Álvarez Isabel, Fernández Iván, Sawadogo Laya, Tamboura Hamidou H, Goyache Félix
INERA, 04 BP 8645, Ouagadougou 04, Burkina Faso.
Trop Anim Health Prod. 2012 Oct;44(7):1469-72. doi: 10.1007/s11250-012-0088-2.
A total of 162 individuals, belonging to three Burkinabé and one Niger sheep populations, were analysed for prion protein (PrP) gene polymorphism at codons 136, 154 and 171. The ARQ allele was the most frequent in both the Burkinabé (86.7%) and the Niger (67.5%) sheep populations. The highly sensitive allele VRQ was not found in the sampled individuals. The highly resistant ARR allele was in very low frequency in the Burkina-Sahel (4.4%) and Mossi (3.2%) populations and was not present in the Djallonké and Touareg populations. Only 4 out of 15 possible PrP genotypes were identified in the sampled individuals. No favourable ARR/ARR genotypes were found in either of the breeds. Sequencing a subgroup of the samples allowed the identification of other five polymorphisms on the PrP gene sequence at codons 116, 138, 151, 237 and 240. The very low frequency of the ARR allele in the West African sheep should dissuade the implementation of a preventive selection programme aimed to increase resistance to scrapie, to avoid an extreme erosion of the genetic stock.
对来自布基纳法索的三个绵羊种群和尼日尔的一个绵羊种群的共162只个体进行了朊病毒蛋白（PrP）基因第136、154和171密码子多态性分析。ARQ等位基因在布基纳法索绵羊种群（86.7%）和尼日尔绵羊种群（67.5%）中最为常见。在所采样个体中未发现高敏性等位基因VRQ。高抗性ARR等位基因在布基纳-萨赫勒种群（4.4%）和莫西种群（3.2%）中的频率很低，在贾隆凯种群和图阿雷格种群中不存在。在所采样个体中仅鉴定出15种可能的PrP基因型中的4种。在任何一个品种中均未发现有利的ARR/ARR基因型。对一部分样本进行测序后，在PrP基因序列的第116、138、151、237和240密码子处鉴定出另外5种多态性。西非绵羊中ARR等位基因的极低频率应会阻止实施旨在提高抗瘙痒病能力的预防性选择计划，以免遗传资源遭到极端侵蚀。
