Kostiainen R, Nokelainen S
Food and Environmental Laboratory, Helsinki, Finland.
J Chromatogr. 1990 Jul 27;513:31-7. doi: 10.1016/s0021-9673(01)89421-8.
A method for the reliable identification of a series of trichothecenes as their trifluoroacetate esters in porridge flake samples is described. The esters were separated by gas chromatography and identified from their retention times relative to n-alkyl-bis(trifluoromethyl)phosphine sulphide (M-series) retention index standards and their electron impact mass spectra. The relative retention times offer an independent identification method by which the reliability of the identification can be improved. The mass spectra and the relative retention times were obtained from the same gas chromatographic-mass spectrometric run. Detection limits were of the order to 0.005-0.05 mg/kg. All the flake samples including oats (ten of fourteen samples studied) contained deoxynivalenol (0.01-0.2 mg/kg) and one oat flake sample contained HT-2 toxin (0.008 mg/kg). The other trichothecenes monitored were not found.
本文描述了一种在燕麦片样品中可靠鉴定一系列单端孢霉烯族毒素三氟乙酸酯的方法。这些酯通过气相色谱法分离，并根据其相对于正烷基双（三氟甲基）硫化膦（M系列）保留指数标准品的保留时间及其电子轰击质谱进行鉴定。相对保留时间提供了一种独立的鉴定方法，可提高鉴定的可靠性。质谱和相对保留时间是在同一次气相色谱 - 质谱运行中获得的。检测限约为0.005 - 0.05 mg/kg。所有薄片样品，包括燕麦（所研究的14个样品中有10个）都含有脱氧雪腐镰刀菌烯醇（0.01 - 0.2 mg/kg），并且一个燕麦片样品含有HT - 2毒素（0.008 mg/kg）。未发现其他监测的单端孢霉烯族毒素。
