Villén T, Abdi Y A, Ericsson O, Gustafsson L L, Sjöqvist F
Department of Clinical Pharmacology, Karolinska Institute, Huddinge University Hospital, Sweden.
J Chromatogr. 1990 Aug 3;529(2):309-17. doi: 10.1016/s0378-4347(00)83837-2.
Analytical methods for determining metrifonate and dichlorvos in whole blood and a sampling procedure suitable for pharmacokinetic studies in man are described. Metrifonate concentrations were determined after chloroform extraction using gas chromatography-nitrogen-phosphorus detection. The within-assay coefficients of variation were 4 and 9% at 19.4 and 0.8 mumol/l (limits of determination), respectively. Dichlorvos was determined using gas chromatography-mass spectrometry of toluene extracts. The within-assay coefficients of variation were 2 and 5% at 225 and 50 nmol/l (limits of determination), respectively. Since both substances are chemically unstable, the blood was collected by dripping it directly from the vein into 0.74 M phosphoric acid.
