Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Biol Pharm Bull. 2012;35(2):184-91. doi: 10.1248/bpb.35.184.
The fatty acid profile of hepatic lipid in spontaneously hypertensive rats (SHR)/NDmcr-cp (cp/cp) rats (SHR/NDcp), which offer an animal model of the metabolic syndrome, was characterized by comparing those in Wistar Kyoto rats (WKY), SHR, stroke-prone spontaneously hypertensive rats (SHRSP) and SHR/NDmcr-cp (+/+) rats (SHR/ND+) . Hierarchical clustering analysis revealed that SHR/NDcp and the other four strains and/or substrains of rats were clearly disparate in fatty acid profile of hepatic lipid and that the disparity observed was due to the drastic increases in the mass of monounsaturated fatty acids, especially palmitoleic acid and oleic acid, in the liver of SHR/NDcp. Activities of stearoyl-CoA desaturase (SCD) and palmitoyl-CoA chain elongase in hepatic microsomes of SHR/NDcp were markedly higher than those of WKY, SHR, SHRSP and SHR/ND+. Activities of palmitoleoyl-CoA chain elongase in the liver of SHR/NDcp were also higher, but to a lesser extent. mRNA levels of SCD1 and elongation of very long-chain fatty acids (Elovl6), but not Elovl5, in the liver of SHR/NDcp were remarkably higher than those of the other four groups of rats. These results suggest that the enhanced expressions of SCD1 and Elovl6 induced abnormalities in fatty acid profile in the liver of SHR/NDcp.
自发性高血压大鼠（SHR）/NDmcr-cp（cp/cp）（SHR/NDcp）的肝脂脂肪酸谱特征，通过比较 Wistar 京都大鼠（WKY）、SHR、易卒中型自发性高血压大鼠（SHRSP）和 SHR/NDmcr-cp（+/+）大鼠（SHR/ND+）的脂肪酸谱来描述。层次聚类分析显示，SHR/NDcp 与其他四个品系和/或亚系大鼠在肝脂脂肪酸谱上明显不同，这种差异是由于 SHR/NDcp 肝脏中单不饱和脂肪酸，特别是棕榈油酸和油酸的质量急剧增加所致。SHR/NDcp 肝微粒体中硬脂酰辅酶 A 去饱和酶（SCD）和棕榈酰辅酶 A 链延长酶的活性明显高于 WKY、SHR、SHRSP 和 SHR/ND+。SHR/NDcp 肝脏中棕榈油酸辅酶 A 链延长酶的活性也较高，但程度较低。SHR/NDcp 肝中 SCD1 和长链脂肪酸延长酶（Elovl6）的 mRNA 水平显著高于其他四组大鼠，而 Elovl5 则没有。这些结果表明，SCD1 和 Elovl6 的表达增强导致 SHR/NDcp 肝脏中脂肪酸谱异常。
