Groenink Lucianne, Verdouw P Monika, van Oorschot Ruud, Olivier Berend
Psychopharmacology, Department of Pharmaceutical Sciences, Utrecht Institute for Pharmaceutical Sciences and Rudolf Magnus Institute of Neuroscience, Utrecht, The Netherlands.
Curr Protoc Pharmacol. 2008 Dec;Chapter 5:Unit 5.18. doi: 10.1002/0471141755.ph0518s43.
Described in this unit is the ultrasonic distress vocalization test in rat pups. This test is a reliable method for detecting anxiolytic properties of test compounds. In this test, ultrasonic vocalizations (30 to 50 kHz) are elicited by separating rat pups of 9 to 11 days of age from their mother and littermates for a brief period of time. The test can be performed under two different stress conditions. Pups are placed in isolation in either a warm (37°C) or cold (18°C) environment for 5 min. The total number and duration of ultrasonic calls emitted by the pups during this period is used as an index of anxiety. Established anxiolytic compounds, including benzodiazepines, serotonin(1A) receptor agonists, and selective serotonin reuptake inhibitors (SSRIs), consistently reduce the number and cumulative duration of these ultrasonic distress vocalizations.
本单元描述的是幼鼠的超声应激发声测试。该测试是检测受试化合物抗焦虑特性的可靠方法。在本测试中，通过将9至11日龄的幼鼠与其母亲和同窝幼崽短暂分离来引发超声发声（30至50千赫）。该测试可在两种不同的应激条件下进行。将幼鼠单独置于温暖（37°C）或寒冷（18°C）环境中5分钟。在此期间幼鼠发出的超声叫声的总数和持续时间用作焦虑指标。已证实的抗焦虑化合物，包括苯二氮䓬类、5-羟色胺（1A）受体激动剂和选择性5-羟色胺再摄取抑制剂（SSRI），可持续减少这些超声应激发声的数量和累积持续时间。
