Yu Zheng, Liu Hong-Ju, Dun Huan-Huan, Dong Qian, Liang Chao
Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2011 Nov;27(4):416-9.
OBJECTIVE: To investigate the molecular mechanisms underlying in the treatment of inflammatory bowel disease by Pulsatilla Decoction. METHODS: Forty Wistar male rats were randomly divided into 5 groups( n = 8)control group, model group, model + positive control group (mesalazine), Pulsatilla Decoction treatment group, in addition, the Pulsatilla Decoction treatment group was divided into middle and high dose group. Intragastric administration was used in the positive control group and Pulsatilla Decoction treatment group. The expression of interleukin-1beta (IL-1beta), interleukin-6(IL-6) and tumor necrosis factor-alpha (TNF-alpha) were detected by real time PCR after extraction of RNA from colons. RESULTS: Compared with the model group, positive medicine and Pulsatilla Decoction group, especially high-dose group, could effectively inhibit the expression of IL-1beta, IL-6 and TNF-alpha. CONCLUSION: Pulsatilla Decoction could exert its effect in the treatment of inflammatory bowel disease by inhibiting the expression of proinflammatory cytokines.
目的：探究白头翁汤治疗炎症性肠病的分子机制。 方法：将40只雄性Wistar大鼠随机分为5组（n = 8），即对照组、模型组、模型+阳性对照组（美沙拉嗪）、白头翁汤治疗组，此外，白头翁汤治疗组再分为中、高剂量组。阳性对照组和白头翁汤治疗组采用灌胃给药。从结肠提取RNA后，通过实时PCR检测白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的表达。 结果：与模型组相比，阳性药物组和白头翁汤组，尤其是高剂量组，能有效抑制IL-1β、IL-6和TNF-α的表达。 结论：白头翁汤可通过抑制促炎细胞因子的表达发挥治疗炎症性肠病的作用。
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