[硫化氢对脂多糖诱导的急性肺损伤大鼠肺表面活性物质的影响]

[Effects of hydrogen sulfide on pulmonary surfactant in rats with acute lung injury induced by lipopolysccharide].

作者信息

Wang Ping, Zhang Jian-Xin, Gong Jian-Pin, Li Lan-Fang, Jin Pu-Le, Ding Cui-Min

机构信息

Department of Respiration, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2011 Nov;27(4):485-9.

PMID:22295533

Abstract

OBJECTIVE

To observe the changes of pulmonary surfactant (PS) in rats with acute lung injury(ALI) induced by lipopolysaccharide (LPS) and to explore the effects of hydrogen sulfide (H2S) on PS.

METHODS

Fourty- eight male rats were randomly divided into six groups (n = 8). They were control group, LPS group, LPS+ NaHS low, middle, high dose groups and LPS+ PPG group. Saline was administrated in Control group. LPS was administrated in LPS group. In LPS + NaHS low, middle, high dose groups or LPS + PPG group, sodium hydrosulfide (NaHS) of different doses or DL-propargylglycine (PPG) were respectively administrated when the rats were administrated of LPS after 3 hours. All the rats were killed at 6 hours after administration of Saline or LPS. The morphological changes of alveolar epithelial type II cells (AEC-II) were respectively observed by transmission electron microscopes. The content of H2S in plasma and activity of cystathionine-gamma-lyase (CSE) in lung tissues were respectively detected. The contents of total protein (TP) and total phospholipids (TPL) in bronchoalveolar lavage fluid (BLAF) were respectively measured. The pulmonary surfactant protein A (SP-A), surfactant protein B (SP-B) and surfactant protein-C (SP-C) mRNA expressions in lung tissues were analysed.

RESULTS

(1) Compared with control group, the content of H2S in plasma, activity of CSE, content of TPL, and SP-A, SP-B and SP-C mRNA expressions were respectively decreased in LPS group (P < 0.05 or P < 0.01). But the content of TP was increased in LPS group (P < 0.01); (2) Compared with LPS group, the content of H2S, activity of CSE and SP-A mRNA expression were significantly increased in LPS + NaHS low, middle and high dose groups (P < 0.05). The SP-B mRNA expression and content of TPL were significantly increased in LPS + NaHS Middle and High dose groups (P < 0.05). The content of TP was decreased in LPS + NaHS High dose group (P < 0.05). The SP-C mRNA expression was not altered in LPS+ NaHS low, middle and high dose groups (P > 0.05); (3) Compared with LPS group, the content of H2S, activity of CSE, content of TPL, and SP-A, SP-B and SP-C mRNA expressions were respectively decreased, but content of TP was increased in LPS + PPG group (P < 0.05).

CONCUSION

The decrease of PS is the important physiopathologic process of ALI induced by LPS. Exogenously applied H2S could attenuate the process of ALI that possibly because H2S could adjust the compose and secretion of PS.

摘要

目的

观察脂多糖（LPS）诱导的急性肺损伤（ALI）大鼠肺表面活性物质（PS）的变化，探讨硫化氢（H₂S）对PS的影响。

方法

48只雄性大鼠随机分为6组（n = 8）。分别为对照组、LPS组、LPS + NaHS低、中、高剂量组和LPS + PPG组。对照组给予生理盐水。LPS组给予LPS。在LPS + NaHS低、中、高剂量组或LPS + PPG组中，大鼠在给予LPS 3小时后分别给予不同剂量的硫氢化钠（NaHS）或DL-炔丙基甘氨酸（PPG）。所有大鼠在给予生理盐水或LPS 6小时后处死。分别用透射电子显微镜观察肺泡Ⅱ型上皮细胞（AEC-II）的形态变化。检测血浆中H₂S含量和肺组织中胱硫醚-γ-裂解酶（CSE）活性。分别测定支气管肺泡灌洗液（BALF）中总蛋白（TP）和总磷脂（TPL）含量。分析肺组织中肺表面活性物质蛋白A（SP-A）、表面活性物质蛋白B（SP-B）和表面活性物质蛋白-C（SP-C）mRNA表达。

结果

（1）与对照组相比，LPS组血浆中H₂S含量、CSE活性、TPL含量以及SP-A、SP-B和SP-C mRNA表达均降低（P < 0.05或P < 0.01）。但LPS组TP含量升高（P < 0.01）；（2）与LPS组相比，LPS + NaHS低、中、高剂量组H₂S含量、CSE活性和SP-A mRNA表达显著升高（P < 0.05）。LPS + NaHS中、高剂量组SP-B mRNA表达和TPL含量显著升高（P < 0.05）。LPS + NaHS高剂量组TP含量降低（P < 0.05）。LPS + NaHS低、中、高剂量组SP-C mRNA表达无变化（P > 0.05）；（3）与LPS组相比，LPS + PPG组H₂S含量、CSE活性、TPL含量以及SP-A、SP-B和SP-C mRNA表达均降低，但TP含量升高（P < 0.05）。