Zhou Jun, Zhou Li-Xiang, Liu Fen-Wu, Ren Yun, Wang Dian-Zhan
Department of Environmental Engineering, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Huan Jing Ke Xue. 2011 Nov;32(11):3400-4.
The dewaterability of cattle biogas slurry facilitated by bioleaching was investigated through batch experiments with co-inoculation of different Acidophilic thiobacilli (Acidithiobacillus thiooxidans TS6 or Acidithiobacillus ferrooxidans LX5). The experiment was set the following 5 treatments: (1) original biogas slurry (CK), (2) 4 g x L(-1) Fe(2+) (uninoculation), (3)2 g x L(-1) S(0) + 25 mL A. t, (4) 4 g x L(-1) Fe(2+) + 25 mL A. f and (5) 2 g x L(-1) S(0) + 4 g x L(-1) Fe(2+) + 12.5 mL A. t + 12.5 mL A. f. During bioleaching, dynamic changes of pH, ORP, Fe(2+), F(3+), total Fe, the settleability, the turbidity of the supernatant after settling for 12 h, and the dewaterability (expressed as specific resistance to filtration gamma or capillary suction time, CST) of biogas slurry were monitored. Results show that specific resistance gamma and CST of bioleached biogas slurry are reduced drastically for the treatments of original biogas slurry spiked with only Fe(2+), the treatment of original biogas slurry co-spiked with Fe(2+) and Acidithiobacillus ferrooxidans LX5, and the treatment of original biogas slurry co-spiked with Fe(2+), S(0) and two Acidophilic thiobacilli. Taking the dewaterability, settleability, the turbidity of the supernatant fluid after settle 12 h and economical cost into account, the treatment of original biogas slurry co-spiked with Fe(2+) and Acidithiobacillus ferrooxidans LX5 is the most suitable pattern for cattle biogas slurry bioleaching. After bioleaching, 1.14% of organic matter, 0.09% of N, 0.05% of P, and 0.1% of K are lost in the bioleaching process, but it don't affect its fertilizer efficiency. Meanwhile, the 63.2% of Cu and 91.3% of Zn are removed from the biogas slurry, and elimination efficiencies of total coliforms in bioleached slurry exceed 99%. This study might provide a new approach for treatment and disposal of biogas slurry.
通过接种不同嗜酸硫杆菌（嗜酸氧化硫硫杆菌TS6或嗜酸氧化亚铁硫杆菌LX5）的批次实验，研究了生物沥浸对牛粪沼液脱水性能的影响。实验设置了以下5种处理方式：（1）原始沼液（CK），（2）4 g·L⁻¹ Fe²⁺（未接种），（3）2 g·L⁻¹ S⁰ + 25 mL嗜酸氧化硫硫杆菌，（4）4 g·L⁻¹ Fe²⁺ + 25 mL嗜酸氧化亚铁硫杆菌，（5）2 g·L⁻¹ S⁰ + 4 g·L⁻¹ Fe²⁺ + 12.5 mL嗜酸氧化硫硫杆菌 + 12.5 mL嗜酸氧化亚铁硫杆菌。在生物沥浸过程中，监测了pH、氧化还原电位（ORP）、Fe²⁺、Fe³⁺、总铁、沉降性能、沉降12 h后上清液的浊度以及沼液的脱水性能（以过滤比阻γ或毛细吸水时间CST表示）的动态变化。结果表明，仅添加Fe²⁺的原始沼液处理、添加Fe²⁺和嗜酸氧化亚铁硫杆菌LX5的原始沼液处理以及添加Fe²⁺、S⁰和两种嗜酸硫杆菌的原始沼液处理，生物沥浸后沼液的过滤比阻γ和CST均大幅降低。综合考虑脱水性能、沉降性能、沉降12 h后上清液的浊度和经济成本，添加Fe²⁺和嗜酸氧化亚铁硫杆菌LX5的原始沼液处理是牛粪沼液生物沥浸最合适的方式。生物沥浸后，生物沥浸过程中损失了1.14%的有机物、0.09%的氮、0.05%的磷和0.1%的钾，但不影响其肥料效率。同时，沼液中63.2%的铜和91.3%的锌被去除，生物沥浸后沼液中总大肠菌群的去除率超过99%。本研究可能为沼液的处理和处置提供一种新方法。
