Department of Thoracic Surgery, Shizuoka Cancer Center, Nagaizumi, Shizuoka, Japan.
Cancer Sci. 2012 Feb;103(2):390-2. doi: 10.1111/j.1349-7006.2011.02136.x.
Echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase (EML4-ALK) and kinesin family member 5B (KIF5B)-ALK are newly identified transforming fusion oncogenes causing non-small-cell lung cancers. These molecular abnormalities have become detectable using not only molecular biological methods, but also highly sensitive immunohistochemistry. During the immunohistochemical study of ALK expression in adenocarcinoma of the lung, we unexpectedly discovered that a small bronchioloalveolar carcinoma (BAC) showed strong ALK immunoreactivity. However, FISH studies failed to reveal EML4-ALK and KIF5B-ALK fusion genes in this BAC. These findings suggest the possibility that a novel or unknown ALK fusion gene plays a crucial role in BAC development.
棘皮动物微管相关蛋白样 4 和间变性淋巴瘤激酶（EML4-ALK）以及驱动蛋白家族成员 5B（KIF5B）-ALK 是新发现的引起非小细胞肺癌的转化融合致癌基因。这些分子异常不仅可以通过分子生物学方法，而且还可以通过高度敏感的免疫组织化学方法来检测。在对肺腺癌中 ALK 表达的免疫组织化学研究中，我们意外地发现一小部分细支气管肺泡癌（BAC）显示出强烈的 ALK 免疫反应性。然而，FISH 研究未能在这种 BAC 中发现 EML4-ALK 和 KIF5B-ALK 融合基因。这些发现提示一种新的或未知的 ALK 融合基因可能在 BAC 的发展中起着关键作用。
