Im K-S, Kim J-H, Kim N-H, Yu C-H, Hur T-Y, Sur J-H
Department of Veterinary Pathology, Small Animal Tumor Diagnostic Center, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Republic of Korea.
J Comp Pathol. 2012 Aug-Oct;147(2-3):121-8. doi: 10.1016/j.jcpa.2011.12.002. Epub 2012 Jan 31.
Epithelial cells adhere tightly to each other by cell-to-cell adhesion and through the basement membrane barrier to prohibit movement. In carcinomas, neoplastic epithelial cells lose their epithelial characteristics and acquire a mesenchymal phenotype during the epithelial-mesenchymal transition (EMT) for invasion and metastasis. The aim of this study was to identify Snail expression and examine the role of Snail protein in canine mammary tumour progression. Immunohistochemical expression of Snail, E-cadherin, oestrogen receptor, human epidermal growth factor receptor-2, cytokeratin 14 and p63 was analyzed in 54 samples of canine mammary epithelial tumours (11 adenomas and 43 carcinomas). Expression of mRNA encoding Snail was evaluated in seven samples (one normal mammary gland, two adenomas and four carcinomas) by reverse transcriptase-polymerase chain reaction. Snail mRNA was detected in all samples. Snail expression correlated significantly with histological type, grade and lymphatic invasion. However, there was no association between Snail expression and molecular subtype and between Snail expression and that of E-cadherin. Snail, a hallmark of EMT, might play an important role in invasion and metastasis of canine mammary carcinomas.
上皮细胞通过细胞间黏附以及穿过基底膜屏障紧密地相互黏附，以阻止移动。在癌组织中，肿瘤上皮细胞在上皮-间质转化（EMT）过程中丧失其上皮特征并获得间充质表型，从而发生侵袭和转移。本研究的目的是确定Snail的表达情况，并检测Snail蛋白在犬乳腺肿瘤进展中的作用。对54例犬乳腺上皮肿瘤样本（11例腺瘤和43例癌）进行了Snail、E-钙黏蛋白、雌激素受体、人表皮生长因子受体-2、细胞角蛋白14和p63的免疫组化表达分析。通过逆转录-聚合酶链反应对7个样本（1个正常乳腺、2个腺瘤和4个癌）中编码Snail的mRNA表达进行了评估。所有样本均检测到Snail mRNA。Snail表达与组织学类型、分级和淋巴侵袭显著相关。然而，Snail表达与分子亚型之间以及Snail表达与E-钙黏蛋白表达之间均无关联。Snail作为EMT的一个标志，可能在犬乳腺癌的侵袭和转移中起重要作用。
