泰国耐氧氟沙星结核分枝杆菌临床分离株中的gyrA和gyrB突变
gyrA and gyrB mutations in ofloxacin-resistant Mycobacterium tuberculosis clinical isolates in Thailand.
作者信息
Pitaksajjakul Pannamthip, Worakhunpiset Suwalee, Chaiprasert Angkana, Boonyasopun Jirakarn, Ramasoota Pongrama
机构信息
Center of Excellence for Antibody Research (CEAR), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
出版信息
Southeast Asian J Trop Med Public Health. 2011 Sep;42(5):1163-7.
In order to identify mutations in gyrA and gyrB genes in 92 ofloxacin-resistant Mycobacterium tuberculosis (OFXr-MTB) clinical isolates collected from Siriraj Hospital, Mahidol University and Chest Disease Institute, Thailand. The quinolone resistance-determining regions (QRDR) of gyrA and gyrB in all 92 OFXr-MTB isolates were amplified using polymerase chain reaction and sequenced. There were 70 isolates with point mutations associated with ofloxacin resistance. In gyrA QRDR, 69 isolates had mutations in gyrA Gly88 (Ala/(75), Ala90 (Val), Ser91 (Pro) and Asp94 (Gly/Ala/His/Asn), the latter being the most common (42%). Only one isolate was found with mutation at position Asp495 (Asn). The other 22 isolates had no mutations in both gyrA and gyrB QRDR. Thus, point mutations in gyrA and gyrB QRDR were responsible for OFXr-MTB clinical isolates in Thailand.
为鉴定从泰国玛希隆大学诗里拉吉医院和胸病研究所收集的92株耐氧氟沙星结核分枝杆菌（OFXr-MTB）临床分离株中gyrA和gyrB基因的突变情况。采用聚合酶链反应扩增所有92株OFXr-MTB分离株中gyrA和gyrB的喹诺酮耐药决定区（QRDR）并进行测序。有70株分离株存在与氧氟沙星耐药相关的点突变。在gyrA QRDR中，69株分离株在gyrA Gly88（Ala/(75)）、Ala90（Val）、Ser91（Pro）和Asp94（Gly/Ala/His/Asn）位点发生突变，后者最为常见（42%）。仅发现1株分离株在Asp495（Asn）位点发生突变。另外22株分离株的gyrA和gyrB QRDR均无突变。因此，gyrA和gyrB QRDR中的点突变是泰国OFXr-MTB临床分离株耐药的原因。
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