来自尼日利亚和巴西的疟原虫分离株中 pfcr 和 pfmdr1 多态性的不同模式：抗疟药物选择压力的潜在作用。
Different patterns of pfcrt and pfmdr1 polymorphisms in P. falciparum isolates from Nigeria and Brazil: the potential role of antimalarial drug selection pressure.
机构信息
Malaria Research Laboratories, Institute for Advanced Medical Research and Training, College of Medicine, University of Ibadan, Nigeria.
出版信息
Am J Trop Med Hyg. 2012 Feb;86(2):211-3. doi: 10.4269/ajtmh.2012.11-0368.
Abstract
The effect of antimalarial drug selection on pfcrt and pfmdr1 polymorphisms in Plasmodium falciparum isolates from two distinct geographical locations was determined in 70 and 18 P. falciparum isolates from Nigeria and Brazil, respectively, using nested polymerase chain reaction and direct DNA sequencing approaches. All isolates from Brazil and 72% from Nigeria harbored the mutant SVMNT and CVIET pfcrt haplotype, respectively. The pfcrt CVMNT haplotype was also observed in (7%) of the Nigerian samples. One hundred percent (100%) and 54% of the parasites from Brazil and Nigeria, respectively, harbored wild-type pfmdr1Asn86. We provide first evidence of emergence of the CVMNT haplotype in West Africa. The high prevalence of pfcrt CVIET and SVMNT haplotypes in Nigeria and Brazil, respectively, is indicative of different selective pressure by chloroquine and amodiaquine. Continuous monitoring of pfcrt SVMNT haplotype is required in endemic areas of Africa, where artesunate-amodiaquine combination is used for treatment of acute uncomplicated malaria.
摘要
采用巢式聚合酶链反应和直接 DNA 测序方法，分别在来自尼日利亚和巴西的 70 和 18 个疟原虫分离株中，检测了抗疟药物选择对疟原虫 falciparum 分离株 pfcrt 和 pfmdr1 多态性的影响。所有来自巴西的分离株和 72%来自尼日利亚的分离株分别携带突变 SVMNT 和 CVIET pfcrt 单倍型。尼日利亚样本中也观察到了 pfcrt CVMNT 单倍型（7%）。来自巴西和尼日利亚的寄生虫分别有 100%和 54%携带野生型 pfmdr1Asn86。我们首次提供了西非人 CVMNT 单倍型出现的证据。尼日利亚和巴西分别存在 pfcrt CVIET 和 SVMNT 单倍型的高流行率，表明氯喹和阿莫地喹的选择压力不同。在使用青蒿琥酯-阿莫地喹联合治疗急性无并发症疟疾的非洲流行地区，需要持续监测 pfcrt SVMNT 单倍型。
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Plasmodium falciparum isolates from Angola show the StctVMNT haplotype in the pfcrt gene.安哥拉的恶性疟原虫分离株在 pfcr t 基因中显示 StctVMNT 单倍型。
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Activities of amodiaquine, artesunate, and artesunate-amodiaquine against asexual- and sexual-stage parasites in falciparum malaria in children.阿莫地喹、青蒿琥酯及青蒿琥酯-阿莫地喹对儿童恶性疟原虫无性和有性阶段寄生虫的活性。
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