Zavrnev S K, Grigorian G L, Minchenkova L E
Mol Biol (Mosk). 1976 Nov-Dec;10(6):1387-93.
The binding of ethidium bromide and acriflavin dyes with DNA modified with a spin-labelled analogue of ethylene imine has been studied. These spin-labels were shown to bind covalently to DNA, at the same time the number of the dye molecules bound is decreased without any changes in the binding constant. Analysis of ESR spectra of the samples in the frozen 50% water-glycerol solution at 77 degrees K for spin-labelled DNA has shown that addition of the dyes increases distance between the labels. This fact might be explained by an increase in DNA length upon formation of the complex with dye molecules.
研究了溴化乙锭和吖啶黄素染料与用乙烯亚胺自旋标记类似物修饰的DNA的结合情况。这些自旋标记物被证明能与DNA共价结合，同时结合的染料分子数量减少，而结合常数没有任何变化。对在77K下冷冻的50%水-甘油溶液中自旋标记DNA样品的电子顺磁共振光谱分析表明，添加染料会增加标记物之间的距离。这一事实可能是由于与染料分子形成复合物时DNA长度增加所致。
